RAT SKELETAL-MUSCLE MITOCHONDRIAL [CA-2+] AND INJURY FROM DOWNHILL WALKING

The purpose of this study was to evaluate the relationship between mitochondrial Ca2+ concentration (MCC) and the extent of muscle injury in rats that have performed prolonged downhill walking (eccentric exercise). MCC was used as an indicator of elevated [Ca2+] in the muscles, and injury was estimated from histochemical analysis of muscle cross sections by determining the numbers of intact fibers per unit area in the muscles. Elevations in MCC in the soleus and vastus intermedius muscles over time postexercise were inversely related (P < 0.05) to the number of intact fibers per square millimeter in the respective muscles after downhill walking. Verapamil administration attenuated the elevation in MCC and injury in histochemical sections resulting from the downhill walking in soleus muscle, but intraperitoneal injection of the chelators EDTA or ethylene glycol-bis(β-aminoethylether)-N,N,N',N'-tetraacetic acid significantly attenuated the increases in MCC and injury to both the vastus intermedius and soleus muscles in the downhill walkers. The chelators appear to exert their 'protective' effects within the specific muscles that show the injury and do not significantly affect serum [Ca2+]. It is concluded that increases in MCC occur during exercise-induced fiber injury and that elevations in cellular Ca2+ may have a role in the etiology of the injury process.