MIGRATORY AND PROLIFERATIVE EFFECT OF PLATELET-DERIVED GROWTH-FACTOR IN RABBIT RETINAL ENDOTHELIAL-CELLS - EVIDENCE OF AN AUTOCRINE PATHWAY OF PLATELET-DERIVED GROWTH-FACTOR

被引：50

作者：

KOYAMA, N ^{[1
]}

WATANABE, S ^{[1
]}

TEZUKA, M ^{[1
]}

MORISAKI, N ^{[1
]}

SAITO, Y ^{[1
]}

YOSHIDA, S ^{[1
]}

机构：

[1] CHIBA UNIV,SCH MED,DEPT INTERNAL MED 2,1-8-1 INOHANA,CHUO KU,CHIBA 260,JAPAN

来源：

JOURNAL OF CELLULAR PHYSIOLOGY | 1994年 / 158卷 / 01期

关键词：

D O I：

10.1002/jcp.1041580102

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Angiogenesis is a crucial event in the progression of diabetic retinopathy. Migration and proliferation of endothelial cells (EC) are important steps in angiogenesis and are caused by angiogenic factors such as basic fibroblast growth factor (bFGF). In this work, capillary EC were isolated from rabbit retinal tissues and rabbit retinal EC (RREC) were found to secrete a migration factor for RREC in conditioned medium (CM). The activity was inhibited by an anti-platelet-derived growth factor (PDGF) antibody, but not by an anti-bFGF antibody. We also found that RREC showed a migratory response to PDGF. The response was induced by PDGF-BB and PDGF-AB dose dependently, but not by PDGF-AA, indicating that it was mediated by PDGF-beta receptor-dependent pathways, and that the PDGF-like factor was PDGF-BB or -AB. In addition, PDGF-BB induced the proliferation of RREC as well as bFGF. These data indicate that RREC have an autocrine pathway of PDGF by the secretion of and the response to PDGF. PDGF may play significant parts in angiogenesis in the progression of diabetic retinopathy. (C) 1994 Wiley-Liss, Inc.

引用

页码：1 / 6

页数：6

共 45 条

[1] THE EFFECTS OF PLATELET-DERIVED GROWTH-FACTOR IN CULTURED MICROVESSEL ENDOTHELIAL-CELLS [J].

BAR, RS ;

BOES, M ;

BOOTH, BA ;

DAKE, BL ;

HENLEY, S ;

HART, MN .

ENDOCRINOLOGY, 1989, 124 (04) :1841-1848

[2] HUMAN MICROVASCULAR ENDOTHELIAL-CELLS EXPRESS RECEPTORS FOR PLATELET-DERIVED GROWTH-FACTOR [J].

BEITZ, JG ;

KIM, IS ;

CALABRESI, P ;

FRACKELTON, AR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (05) :2021-2025

[3] CHARACTERIZATION OF RELEASE OF BASIC FIBROBLAST GROWTH-FACTOR FROM BOVINE RETINAL ENDOTHELIAL-CELLS IN MONOLAYER-CULTURES [J].

BROOKS, RA ;

BURRIN, JM ;

KOHNER, EM .

BIOCHEMICAL JOURNAL, 1991, 276 :113-120

[4] GROWTH-FACTOR EFFECTS ON CELLS OF THE VASCULAR WALL - A SURVEY [J].

DAMORE, PA ;

SMITH, SR .

GROWTH FACTORS, 1993, 8 (01) :61-75

[5] CULTURED ENDOTHELIAL-CELLS PRODUCE A PLATELET-DERIVED GROWTH FACTOR-LIKE PROTEIN [J].

DICORLETO, PE ;

BOWENPOPE, DF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (07) :1919-1923

[6] PDGF ALPHA-RECEPTORS AND BETA-RECEPTORS ACTIVATE UNIQUE AND COMMON SIGNAL TRANSDUCTION PATHWAYS [J].

ERIKSSON, A ;

SIEGBAHN, A ;

WESTERMARK, B ;

HELDIN, CH ;

CLAESSONWELSH, L .

EMBO JOURNAL, 1992, 11 (02) :543-550

[7] ANGIOGENIC FACTORS [J].

FOLKMAN, J ;

KLAGSBRUN, M .

SCIENCE, 1987, 235 (4787) :442-447

[8]

FOLKMAN J, 1992, J BIOL CHEM, V267, P10931

[9]

FUNA K, 1990, CANCER RES, V50, P748

[10] DIFFERENTIAL BINDING, BIOLOGICAL AND BIOCHEMICAL ACTIONS OF RECOMBINANT PDGF AA, AB, AND BB MOLECULES ON CONNECTIVE-TISSUE CELLS [J].

GROTENDORST, GR ;

IGARASHI, A ;

LARSON, R ;

SOMA, Y ;

CHARETTE, M .

JOURNAL OF CELLULAR PHYSIOLOGY, 1991, 149 (02) :235-243

← 1 2 3 4 5 →