AN INVESTIGATION BY EIS OF GRAMICIDIN CHANNELS IN BILAYER-LIPID MEMBRANES

被引:22
作者
ALONSOROMANOWSKI, S
GASSA, LM
VILCHE, JR
机构
[1] NATL UNIV LA PLATA,FAC CIENCIAS EXACTAS,INST INVEST FISICOQUIM TEOR & APLICADAS,RA-1900 LA PLATA,ARGENTINA
[2] UNIV NACL QUILMES,DEPT CIENCIA & TECNOL,RA-1876 QUILMES,ARGENTINA
关键词
BLM; EIS; GRAMACIDINA; IONIC TRANSFER; CHANNEL MEMBRANE CONTRIBUTIONS;
D O I
10.1016/0013-4686(95)00036-E
中图分类号
O646 [电化学、电解、磁化学];
学科分类号
081704 ;
摘要
The influence of Li+, Na+, K+, and Cs+ ions on the characteristics and properties of Black Lipid Membranes (BLM) made of diphytanoylphosphatydilcholine (DPhPC), with thickness thinner than 10 nm, were investigated using electrochemical impedance spectroscopy (EIS). The impedance spectra of the base system including the film and its adjacent chloride ion containing aqueous solutions exhibited for all tested cations a single capacitive contribution. The corresponding time constant can be discussed in terms of a model which includes a dielectric dispersion in the membrane. BLM films doped with a low concentration of Gramicidin A have exhibited values of conductance in contact with electrolytes containing different cations at low aqueous concentration of Gramicidin A, that indicate ionic transference from one side to the other of the membrane. This phenomenon was detected by using either dc or ac voltage perturbations. Data obtained using EIS techniques show two time constants, which can be assigned to the membrane and to the channel/membrane contributions, respectively.
引用
收藏
页码:1561 / 1567
页数:7
相关论文
共 32 条
[1]  
ANDERSEN OS, 1984, ANNU REV PHYSIOL, V46, P531
[2]   CHANNEL FORMATION KINETICS OF GRAMICIDIN-A IN LIPID BILAYER MEMBRANES [J].
BAMBERG, E ;
LAUGER, P .
JOURNAL OF MEMBRANE BIOLOGY, 1973, 11 (02) :177-194
[3]   THE APPLICATION OF ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY AND IDENTIFICATION PROCEDURES TO THE INVESTIGATION OF THE DISSOLUTION AND PASSIVATION OF IRON IN CARBONATE-BICARBONATE BUFFERS AT 25-DEGREES-C [J].
CASTRO, EB ;
REAL, SG ;
MILOCCO, RH ;
VILCHE, JR .
ELECTROCHIMICA ACTA, 1991, 36 (01) :117-126
[4]  
CASTRO EB, 1989, MATER SCI FORUM, V44, P417
[5]   MOLECULAR-ORGANIZATION OF BIMOLECULAR LIPID-MEMBRANES - STUDY OF LOW FREQUENCY MAXWELL-WAGNER IMPEDANCE DISPERSION [J].
COSTER, HGL ;
SMITH, JR .
BIOCHIMICA ET BIOPHYSICA ACTA, 1974, 373 (02) :151-164
[6]   EFFECT OF BENZYL ALCOHOL ON LIPID BILAYERS - COMPARISON OF BILAYER SYSTEMS [J].
EBIHARA, L ;
HALL, JE ;
MACDONALD, RC ;
MCINTOSH, TJ ;
SIMON, SA .
BIOPHYSICAL JOURNAL, 1979, 28 (02) :185-196
[7]   THICKNESS, COMPOSITION AND STRUCTURE OF SOME LIPID BILAYERS AND NATURAL MEMBRANES [J].
FETTIPLACE, R ;
ANDREWS, DM ;
HAYDON, DA .
JOURNAL OF MEMBRANE BIOLOGY, 1971, 5 (03) :277-+
[8]   AN IMPEDANCE SPECTROSCOPY STUDY OF THE ANODICALLY FORMED BARRIER LAYER ON ALUMINUM SUBSTRATES [J].
GERVASI, CA ;
VILCHE, JR .
ELECTROCHIMICA ACTA, 1992, 37 (08) :1389-1394
[9]  
Haruyama S., 1987, ELECTROCHEM SOC P, V87, P197
[10]   DISCRETENESS OF CONDUCTANCE CHANGE IN BIMOLECULAR LIPID MEMBRANES IN PRESENCE OF CERTAIN ANTIBIOTICS [J].
HLADKY, SB ;
HAYDON, DA .
NATURE, 1970, 225 (5231) :451-&