IDENTIFICATION OF NOVEL CDNAS ENCODING HUMAN KEXIN-LIKE PROTEASE, PACE4 ISOFORMS

被引：37
作者：
TSUJI, A
HIGASHINE, K
HINE, C
MORI, K
TAMAI, Y
NAGAMUNE, H
MATSUDA, Y
机构：
[1] Univ Tokushima, Fac Engn, Dept Biol Sci and Technol, Tokushima
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 200卷 / 02期
关键词：
D O I：
10.1006/bbrc.1994.1541
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
PACE4 has been identified as a second human subtilisin-like protease by Keifer et al. [DNA and Cell Biology (1991) 10, 757-769]. In this study, we isolated two novel cDNAs coding for PACE4 isoforms (PACE4C and PACE4D) from a human placenta cDNA library. The deduced PACE4C protein sequence (652 amino acids) lacks the cysteine-rich region located at the carboxy terminus of PACE4. The DNA sequence of the 3'-untranslated region (1 kb) of PACE4C is not homologous to the PACE4 sequence. The protein (497 amino acids) encoded by PACE4D cDNA lacks a signal peptide, a propeptide and the cysteine-rich region. These isoform cDNAs were also isolated from rat pituitary cDNA library. (C) 1994 Academic Press, Inc.
引用
收藏
页码：943 / 950
页数：8
相关论文
共 18 条
[11]   A MEMBRANE-BOUND, CALCIUM-DEPENDENT PROTEASE IN YEAST ALPHA-CELL CLEAVING ON THE CARBOXYL SIDE OF PAIRED BASIC RESIDUES [J].
MIZUNO, K ;
NAKAMURA, T ;
TAKADA, K ;
SAKAKIBARA, S ;
MATSUO, H .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1987, 144 (02) :807-814
[12]   YEAST KEX2 GENE ENCODES AN ENDOPEPTIDASE HOMOLOGOUS TO SUBTILISIN-LIKE SERINE PROTEASES [J].
MIZUNO, K ;
NAKAMURA, T ;
OHSHIMA, T ;
TANAKA, S ;
MATSUO, H .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 156 (01) :246-254
[13]   IDENTIFICATION AND FUNCTIONAL EXPRESSION OF A NEW MEMBER OF THE MAMMALIAN KEX2-LIKE PROCESSING ENDOPROTEASE FAMILY - ITS STRIKING STRUCTURAL SIMILARITY TO PACE4 [J].
NAKAGAWA, T ;
HOSAKA, M ;
TORII, S ;
WATANABE, T ;
MURAKAMI, K ;
NAKAYAMA, K .
JOURNAL OF BIOCHEMISTRY, 1993, 113 (02) :132-135
[14]   IDENTIFICATION OF AN ISOFORM WITH AN EXTREMELY LARGE CYS-RICH REGION OF PC6, A KEX2-LIKE PROCESSING ENDOPROTEASE [J].
NAKAGAWA, T ;
MURAKAMI, K ;
NAKAYAMA, K .
FEBS LETTERS, 1993, 327 (02) :165-171
[15]  
NAKAYAMA K, 1992, J BIOL CHEM, V267, P5897
[16]   CDNA SEQUENCE OF 2 DISTINCT PITUITARY PROTEINS HOMOLOGOUS TO KEX2 AND FURIN GENE-PRODUCTS - TISSUE-SPECIFIC MESSENGER-RNAS ENCODING CANDIDATES FOR PRO-HORMONE PROCESSING PROTEINASES [J].
SEIDAH, NG ;
GASPAR, L ;
MION, P ;
MARCINKIEWICZ, M ;
MBIKAY, M ;
CHRETIEN, M .
DNA AND CELL BIOLOGY, 1990, 9 (06) :415-424
[17]   IDENTIFICATION OF A CDNA-ENCODING A 2ND PUTATIVE PROHORMONE CONVERTASE RELATED TO PC2 IN ATT20 CELLS AND ISLETS OF LANGERHANS [J].
SMEEKENS, SP ;
AVRUCH, AS ;
LAMENDOLA, J ;
CHAN, SJ ;
STEINER, DF .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (02) :340-344
[18]  
SMEEKENS SP, 1990, J BIOL CHEM, V265, P2997
12