IDENTIFICATION OF NOVEL CDNAS ENCODING HUMAN KEXIN-LIKE PROTEASE, PACE4 ISOFORMS

被引：37
作者：
TSUJI, A
HIGASHINE, K
HINE, C
MORI, K
TAMAI, Y
NAGAMUNE, H
MATSUDA, Y
机构：
[1] Univ Tokushima, Fac Engn, Dept Biol Sci and Technol, Tokushima
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 200卷 / 02期
关键词：
D O I：
10.1006/bbrc.1994.1541
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
PACE4 has been identified as a second human subtilisin-like protease by Keifer et al. [DNA and Cell Biology (1991) 10, 757-769]. In this study, we isolated two novel cDNAs coding for PACE4 isoforms (PACE4C and PACE4D) from a human placenta cDNA library. The deduced PACE4C protein sequence (652 amino acids) lacks the cysteine-rich region located at the carboxy terminus of PACE4. The DNA sequence of the 3'-untranslated region (1 kb) of PACE4C is not homologous to the PACE4 sequence. The protein (497 amino acids) encoded by PACE4D cDNA lacks a signal peptide, a propeptide and the cysteine-rich region. These isoform cDNAs were also isolated from rat pituitary cDNA library. (C) 1994 Academic Press, Inc.
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页码：943 / 950
页数：8
相关论文
共 18 条
[1]   MAMMALIAN SUBTILISINS - THE LONG-SOUGHT DIBASIC PROCESSING ENDOPROTEASES [J].
BARR, PJ .
CELL, 1991, 66 (01) :1-3
[2]  
EIPPER BA, 1992, J BIOL CHEM, V267, P4008
[3]  
EIPPER BA, 1990, TRENDS ENDOCRIN MET, V1, P388
[4]   INTRACELLULAR TARGETING AND STRUCTURAL CONSERVATION OF A PROHORMONE-PROCESSING ENDOPROTEASE [J].
FULLER, RS ;
BRAKE, AJ ;
THORNER, J .
SCIENCE, 1989, 246 (4929) :482-486
[5]   ISOLATION OF 2 COMPLEMENTARY DEOXYRIBONUCLEIC-ACID CLONES FROM A RAT INSULINOMA CELL-LINE BASED ON SIMILARITIES TO KEX2 AND FURIN SEQUENCES AND THE SPECIFIC LOCALIZATION OF EACH TRANSCRIPT TO ENDOCRINE AND NEUROENDOCRINE TISSUES IN RATS [J].
HAKES, DJ ;
BIRCH, NP ;
MEZEY, A ;
DIXON, JE .
ENDOCRINOLOGY, 1991, 129 (06) :3053-3063
[6]  
HATSUZAWA K, 1992, KYOIOCH, V111, P296
[7]   ISOLATION OF THE PUTATIVE STRUCTURAL GENE FOR THE LYSINE-ARGININE-CLEAVING ENDOPEPTIDASE REQUIRED FOR PROCESSING OF YEAST PREPRO-ALPHA-FACTOR [J].
JULIUS, D ;
BRAKE, A ;
BLAIR, L ;
KUNISAWA, R ;
THORNER, J .
CELL, 1984, 37 (03) :1075-1089
[8]  
KEIFER MC, 1991, DNA CELL BIOL, V10, P757
[9]   PROTEOLYSIS IN NEUROPEPTIDE PROCESSING AND OTHER NEURAL FUNCTIONS [J].
LOH, YP ;
BROWNSTEIN, MJ ;
GAINER, H .
ANNUAL REVIEW OF NEUROSCIENCE, 1984, 7 :189-222
[10]   CDNA STRUCTURE OF THE MOUSE AND RAT SUBTILISIN KEXIN-LIKE PC5 - A CANDIDATE PROPROTEIN CONVERTASE EXPRESSED IN ENDOCRINE AND NONENDOCRINE CELLS [J].
LUSSON, J ;
VIEAU, D ;
HAMELIN, J ;
DAY, R ;
CHRETIEN, M ;
SEIDAH, NG .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (14) :6691-6695
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