ENZYME-IMMUNOASSAY FOR FLECAINIDE

被引：2

作者：

FRECHE, JP

COUDERT, G

WELLMAN, M

SIEST, G

机构：

[1] CTR MEDICAMENT,CNRS,URA 597,30 RUE LIONNOIS,F-54000 NANCY,FRANCE

[2] SOC STABILIGEN,CTR VIE PARC ACT NANCY BRABOIS,VANDOEUVRE NANCY,FRANCE

来源：

CLINICA CHIMICA ACTA | 1990年 / 190卷 / 03期

关键词：

Enzyme immunoassay; Flecainide; Flecainide-hemifumarate-horseradish peroxidase; Flecainide-hemisuccinate-bovine serum albumin;

D O I：

10.1016/0009-8981(90)90167-Q

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

In order to develop an enzyme immunoassay (ELISA) for measurement of plasma flecainide levels, we first synthesized flecainide-hemisuccinate-BSA as immunogen. A highly specific antiserum was then raised in rabbits. Enzyme-labelled flecainide was prepared by the mixed anhydride method using hemifumarate-flecainide and horse radish peroxidase. The limit of detection was 10 ng/ml, the method was linear up to 1000 μg/l. The intra and inter-assay variation was 5.1-7.8%. Cross reactivity with various metabolites of flecainide or some chemically related drugs was < 0.1%. By contrast the cross reaction with some structural analogues achieved 25%. The importance of parts of the aromatic ring structure in immunogenic properties of this drug is discussed. © 1990.

引用

页码：139 / 146

页数：8