REPLACEMENT RECOMBINATION IN CORYNEFORM BACTERIA - HIGH-EFFICIENCY INTEGRATION REQUIREMENT FOR NONMETHYLATED PLASMID DNA

被引：33

作者：

VERTES, AA ^{[1
]}

HATAKEYAMA, K ^{[1
]}

INUI, M ^{[1
]}

KOBAYASHI, M ^{[1
]}

KURUSU, Y ^{[1
]}

YUKAWA, H ^{[1
]}

机构：

[1] MITSUBISHI PETROCHEM CO LTD, TSUKUBA RES CTR, INASHIKI, IBARAKI 30003, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1993年 / 57卷 / 12期

关键词：

D O I：

10.1271/bbb.57.2036

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a versatile system that permits efficient manipulation of coryneform bacteria by gene disruption and replacement. Circumventing the methyl-specific restriction system in certain of these bacteria by using non-methylated plasmid DNA was necessary. Integrative plasmid DNA containing an in vitro disrupted copy of the Brevibacterium flavum thrB gene was introduced into B. flavum at an efficiency of 2.4 x 10(2) integrants/mug. Integration occurred by homologous recombination either via a Campbell-like mechanism or via a double crossover event. Integrants were stable for over 32 generations.

引用

页码：2036 / 2038

页数：3

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