DETERMINATION OF THE NUMBER OF THIOETHER-LINKED CYSTEINE RESIDUES IN CYTOCHROMES-C AND PHYCOBILIPROTEINS

被引：33

作者：

GLAZER, AN ^{[1
]}

HIXSON, CS ^{[1
]}

DELANGE, RJ ^{[1
]}

机构：

[1] UNIV CALIF LOS ANGELES,SCH MED,DEPT BIOL CHEM,LOS ANGELES,CA 90024

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 92卷 / 02期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/0003-2697(79)90689-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Hydrolysis of cytochromes c and phycobiliproteins in 6 n HCl containing 0.21 m dimethylsulfoxide at 110°C for 22 h results in the quantitative conversion of cysteinyl, cystinyl, and thioether-linked cysteinyl residues to cysteic acid. Methionine is converted to homocysteic acid in a 10% yield. The number of thioether-linked cysteinyl residues can be calculated, if the number of cysteinyl and cystinyl residues is determined separately as S-carboxymethylcysteine in a 6 n HCl hydrolyzate of reduced, carboxymethylated protein. Data are presented on the number of thioether-linked cysteinyl residues present in R-, B-, and C-phycoerythrins, C- and R-phycocyanins, allophycocyanins, and the subunits of these proteins. © 1979.

引用

页码：489 / 496

页数：8

共 28 条

[1]

BRYANT DA, 1978, J BIOL CHEM, V253, P220

[2] CHARACTERIZATION AND STRUCTURAL-PROPERTIES OF MAJOR BILIPROTEINS OF ANABAENA SP [J].

BRYANT, DA ;

GLAZER, AN ;

EISERLING, FA .

ARCHIVES OF MICROBIOLOGY, 1976, 110 (01) :61-75

[3]

BRYANT DA, 1977, THESIS U CALIFORNIA

[4] CHROMOPHORE-CONTAINING PEPTIDE SEQUENCES IN C-PHYCOCYANIN FROM MASTIGOCLADUS-LAMINOSUS [J].

BYFIELD, PGH ;

ZUBER, H .

FEBS LETTERS, 1972, 28 (01) :36-&

[5]

CRESTFIELD AM, 1963, J BIOL CHEM, V238, P622

[6] AMINO-ACID SEQUENCE OF FLAGELLIN FROM BACILLUS-SUBTILIS 168 - COMPARISON WITH OTHER BACTERIAL FLAGELLINS [J].

DELANGE, RJ ;

CHANG, JY ;

SHAPER, JH ;

MARTINEZ, RJ ;

KOMATSU, SK ;

GLAZER, AN .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1973, 70 (12) :3428-3431

[7] AMINO ACID COMPOSITION, HEME CONTENT, AND MOLECULAR WEIGHT OF CYTOCHROME-C3 OF DESULFOVIBRIO-DESULFURICANS AND DESULFOVIBRIO-VULGARIS [J].

DRUCKER, H ;

TROUSIL, EB ;

BARLOW, GH ;

MARGOLIASH, E .

BIOCHEMISTRY, 1970, 9 (07) :1515-+

[8] DECOMPOSITION OF DL-METHIONINE SULFOXIDE IN 6 N HYDROCHLORIC ACID [J].

FLOYD, NF ;

CAMMAROTI, M ;

LAVINE, TF .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1963, 102 (03) :343-&

[9]

GLAZER AN, 1977, J BIOL CHEM, V252, P32

[10]

GLAZER AN, 1973, J BIOL CHEM, V248, P663

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