DETERMINATION OF SPECTINOMYCIN BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUOROMETRIC DETECTION

被引：31
作者：
MYERS, HN
RINDLER, JV
机构：
[1] The Upjohn Company, Kalamazoo
来源：
JOURNAL OF CHROMATOGRAPHY | 1979年 / 176卷 / 01期
关键词：
D O I：
10.1016/S0021-9673(00)92090-9
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A high-performance liquid chromatographic method for the quantitative determination of spectinomycin in the presence of actinospectinoic acid and actinamine is described. A combination of paired ion chromatography, post column oxidation, post column derivatization of fluorometric detection is employeed. Total assay time is less than 15 min. The assay was designed for determining spectinomycin in samples for spectinomycin finished products, process samples and fermentation beers. Quantitative results from this procedure are compared to those obtained by a turbidimetric microbiological assay and are in good agreement. © 1979.
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页码：103 / 108
页数：6
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