ANALYSIS OF DEZOCINE IN SERUM AND URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND PRECOLUMN DERIVATIZATION

被引：1
作者：
WILSON, JM
COHEN, RI
KEZER, EA
SMITH, ER
机构：
[1] UNIV MASSACHUSETTS,MED CTR,DEPT ANESTHESIOL,WORCESTER,MA 01655
[2] UNIV MASSACHUSETTS,MED CTR,DEPT PHARMACOL,WORCESTER,MA 01655
来源：
JOURNAL OF LIQUID CHROMATOGRAPHY | 1994年 / 17卷 / 19期
关键词：
D O I：
10.1080/10826079408013614
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We present a reverse phase liquid chromatographic assay involving pre-column o-phthalaldehyde derivatization and fluorescence detection with the capability of measuring dezocine in human serum and urine for pharmacokinetic or forensic purposes. It features a simple liquid-liquid extraction scheme using hexane, conservative detection limits of 1 ng/ml, linearity to 1000 ng/ml, precision of 10% (CV%) in the low ng/ml region, approximate chromatography time of 25 minutes and the use of a new internal standard, WY-19083.
引用
收藏
页码：4245 / 4257
页数：13
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