NUCLEASE SENSITIVITY AND FUNCTIONAL-ANALYSIS OF A MAIZE HISTONE H3 GENE PROMOTER

被引：9

作者：

BRIGNON, P ^{[1
]}

LEPETIT, M ^{[1
]}

GIGOT, C ^{[1
]}

CHAUBET, N ^{[1
]}

机构：

[1] UNIV STRASBOURG 1, CNRS, INST BIOL MOLEC PLANTES, 12 RUE GEN ZIMMER, F-67070 STRASBOURG, FRANCE

来源：

PLANT MOLECULAR BIOLOGY | 1993年 / 22卷 / 06期

关键词：

CHROMATIN; HISTONE PROMOTER; HYPERSENSITIVE SITES; NUCLEASE ACCESSIBILITY; NUCLEOSOMES; TRANSGENIC PLANTS;

D O I：

10.1007/BF00028973

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A 1 kb region of a maize H3 histone gene promoter has been analysed at a structural and functional level. Micrococcal nuclease digestion of isolated nuclei showed that the promoter region is organized into nucleosomes but a zone extending over approximately one nucleosome (20 to 230 bp upstream of the TATA box) displays remarkable accessibility to digestion. Three DNase I-hypersensitive sites were found within this zone at the vicinity of consensus sequences, some of which are already known to act as cis elements. This promoter region is able to direct faithful expression of the GUS reporter gene in meristematic tissues of transgenic tobacco plants.

引用

页码：1007 / 1015

页数：9