DISSOCIATION OF COLLAGENASE-TISSUE INHIBITOR OF METALLOPROTEINASES-1 (TIMP-1) COMPLEX - ITS APPLICATION FOR THE INDEPENDENT MEASUREMENTS OF TIMP-1 AND COLLAGENASE ACTIVITY IN CRUDE CULTURE MEDIA AND BODY-FLUIDS

被引：7
|
作者：
YAMASHITA, K
ZHANG, J
ZOU, LM
HAYAKAWA, H
NOGUCHI, T
KONDO, I
NARITA, O
FUJIMOTO, N
IWATA, K
HAYAKAWA, T
机构：
[1] AICHI GAKUIN UNIV,SCH DENT,DEPT BIOCHEM,1-100 KUSUMOTO CHO,CHIKUSA KU,NAGOYA 464,JAPAN
[2] AICHI GAKUIN UNIV,SCH DENT,DEPT PERIODONTOL,CHIKUSA KU,NAGOYA 464,JAPAN
[3] NAGOYA UNIV,BRANCH HOSP,SCH MED,DEPT OBSTET & GYNECOL,HIGASHI KU,NAGOYA 461,JAPAN
[4] FUJI CHEM IND CO LTD,TAKAOKA,TOYAMA 933,JAPAN
来源：
MATRIX | 1992年 / 12卷 / 06期
关键词：
ANTI-TIMP-1 MONOCLONAL ANTIBODY-AFFINITY COLUMN; COLLAGENASE ASSAY; DISSOCIATION OF ENZYME-INHIBITOR COMPLEX; INTERSTITIAL COLLAGENASE; TIMP-1; ASSAY; TISSUE INHIBITOR OF METALLOPROTEINASES-1 (TIMP-1);
D O I：
10.1016/S0934-8832(11)80093-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Collagenase-tissue inhibitor of metalloproteinases-1 (TIMP-1) complex was prepared from activated collagenase and TIMP-1 purified from culture media of human skin fibroblasts. After having been confirmed to be a complex by zinc chelate chromatography, the complex was demonstrated to dissociate by passage through an anti-TIMP-1 monoclonal antibody-affinity column. On the basis of above evidence, a simple strategy was set up for the independent measurements of TIMP-1 concentration, and both active and total collagenase activities in crude culture media and body fluids.
引用
收藏
页码：481 / 487
页数：7
相关论文
共 50 条
  • [41] Tissue inhibitor of metalloproteinases 1 (TIMP-1) as an immunohistochemical marker for colorectal cancer
    Sorensen, I.
    Winther, H.
    Foged, N.
    Fenger, C.
    Brunner, N.
    EJC SUPPLEMENTS, 2005, 3 (02): : 196 - 196
  • [42] Elevated serum tissue inhibitor of metalloproteinases-1 (TIMP-1) level predicts shorter survival in ovarian cancer
    Jamshidi, Navid
    Shrivastava, Vikas
    Leitzel, Kim
    Ali, Suhail
    Podczask, Edward
    Macneill, Colin
    Deimling, Timothy
    Brown-Shimer, Sheryl
    Pierce, Karen
    Carney, Walter
    Rizvi, Syed
    Abbi, Kamal
    Lipton, Allan
    CANCER RESEARCH, 2010, 70
  • [43] Elevated serum tissue inhibitor of metalloproteinases-1 (TIMP-1) level predicts shorter survival in ovarian cancer
    Jamshidi, Navid
    Shrivastava, Vikas
    Leitzel, Kim
    Ali, Suhail
    Podczask, Edward
    Macneill, Colin
    Deimling, Timothy
    Brown-Shimer, Sheryl
    Pierce, Karen
    Carney, Walter
    Rizvi, Syed
    Abbi, Kemal
    Lipton, Allan
    CANCER RESEARCH, 2010, 70
  • [44] Inhibitory effect of emodin on tissue inhibitor of metalloproteinases-1 (TIMP-1) expression in rat hepatic stellate cells
    Gui, Min
    Zhang, Yue Fan
    Xiao, Zhen Yu
    Sun, Peng
    Dai, Jian Feng
    Wang, Shuo Feng
    Rui, Yao Cheng
    Zhang, Jun Ping
    DIGESTIVE DISEASES AND SCIENCES, 2007, 52 (01) : 200 - 207
  • [45] Preparation of recombinant tissue inhibitor of metalloproteinases-1 (TIMP-1) in high yield and identification of a hydrophobic surface feature
    Hodges, DJ
    Reid, DG
    Rowan, AD
    Clark, IM
    Cawston, TE
    EUROPEAN JOURNAL OF BIOCHEMISTRY, 1998, 257 (03): : 562 - 569
  • [46] Elevated serum and synovial fluid levels of collagenase, stromelysin and tissue inhibitor of metalloproteinases (TIMP-1) in early inflammatory arthritis.
    Cunnane, G
    Summers, C
    Taylor, D
    Cawston, T
    Bresnihan, B
    FitzGerald, O
    ARTHRITIS AND RHEUMATISM, 1996, 39 (09): : 630 - 630
  • [47] Both tissue inhibitors of metalloproteinases-1 (TIMP-1) and TIMP-2 activate Ras but through different pathways
    Wang, T
    Yamashita, K
    Iwata, K
    Hayakawa, T
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2002, 296 (01) : 201 - 205
  • [48] Inhibitory Effect of Emodin on Tissue Inhibitor of Metalloproteinases-1 (TIMP-1) Expression in Rat Hepatic Stellate Cells
    Min Gui
    Yue Fan Zhang
    Zhen Yu Xiao
    Peng Sun
    Jian Feng Dai
    Shuo Feng Wang
    Yao Cheng Rui
    Jun Ping Zhang
    Digestive Diseases and Sciences, 2007, 52 : 200 - 207
  • [49] Identification of invariant chain CD74 as a functional receptor of tissue inhibitor of metalloproteinases-1 (TIMP-1)
    Schoeps, Benjamin
    Eckfeld, Celina
    Flueter, Laura
    Keppler, Selina
    Mishrat, Ritu
    Knolle, Percy
    Bayer, Felix
    Boettcher, Jan
    Hermann, Chris D.
    Haeussler, Daniel
    Krueger, Achim
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2021, 297 (03)
  • [50] Expression of tissue inhibitor of matrix metalloproteinases-1 (TIMP-1) and TIMP-3 protein in invasive breast carcinoma: correlations with prognostic factors
    Yin, Yongmei
    BIOMEDICINE & PHARMACOTHERAPY, 2008, 62 (07) : 424 - 425
12345