DISSOCIATION OF COLLAGENASE-TISSUE INHIBITOR OF METALLOPROTEINASES-1 (TIMP-1) COMPLEX - ITS APPLICATION FOR THE INDEPENDENT MEASUREMENTS OF TIMP-1 AND COLLAGENASE ACTIVITY IN CRUDE CULTURE MEDIA AND BODY-FLUIDS

被引：7
|
作者：
YAMASHITA, K
ZHANG, J
ZOU, LM
HAYAKAWA, H
NOGUCHI, T
KONDO, I
NARITA, O
FUJIMOTO, N
IWATA, K
HAYAKAWA, T
机构：
[1] AICHI GAKUIN UNIV,SCH DENT,DEPT BIOCHEM,1-100 KUSUMOTO CHO,CHIKUSA KU,NAGOYA 464,JAPAN
[2] AICHI GAKUIN UNIV,SCH DENT,DEPT PERIODONTOL,CHIKUSA KU,NAGOYA 464,JAPAN
[3] NAGOYA UNIV,BRANCH HOSP,SCH MED,DEPT OBSTET & GYNECOL,HIGASHI KU,NAGOYA 461,JAPAN
[4] FUJI CHEM IND CO LTD,TAKAOKA,TOYAMA 933,JAPAN
来源：
MATRIX | 1992年 / 12卷 / 06期
关键词：
ANTI-TIMP-1 MONOCLONAL ANTIBODY-AFFINITY COLUMN; COLLAGENASE ASSAY; DISSOCIATION OF ENZYME-INHIBITOR COMPLEX; INTERSTITIAL COLLAGENASE; TIMP-1; ASSAY; TISSUE INHIBITOR OF METALLOPROTEINASES-1 (TIMP-1);
D O I：
10.1016/S0934-8832(11)80093-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Collagenase-tissue inhibitor of metalloproteinases-1 (TIMP-1) complex was prepared from activated collagenase and TIMP-1 purified from culture media of human skin fibroblasts. After having been confirmed to be a complex by zinc chelate chromatography, the complex was demonstrated to dissociate by passage through an anti-TIMP-1 monoclonal antibody-affinity column. On the basis of above evidence, a simple strategy was set up for the independent measurements of TIMP-1 concentration, and both active and total collagenase activities in crude culture media and body fluids.
引用
收藏
页码：481 / 487
页数：7
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