ABSENCE OF UNSCHEDULED DNA-SYNTHESIS IN RAT HEPATOCYTES TREATED WITH MUTAGENIC AND CYTOTOXIC CHLORINATED HUMIC SUBSTANCES

被引：5

作者：

UBOM, G ^{[1
]}

CHIPMAN, JK ^{[1
]}

机构：

[1] UNIV BIRMINGHAM,SCH BIOCHEM,BIRMINGHAM B15 2TT,W MIDLANDS,ENGLAND

来源：

MUTATION RESEARCH | 1994年 / 321卷 / 1-2期

关键词：

UNSCHEDULED DNA SYNTHESIS; DNA SYNTHESIS; UNSCHEDULED; HEPATOCYTES; HUMIC ACIDS; CHLORINATED; CHMA;

D O I：

10.1016/0165-1218(94)90120-1

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Chlorinated hydrophilic macromolecular humic acids (CHMA) (0.07-3.0 mg per plate) were mutagenic in Salmonella typhimurium strain TA100. In contrast, the chlorinated derivatives did not induce unscheduled DNA synthesis (nuclear incorporation of [H-3]thymidine) in cultured rat hepatocytes even after depletion of intracellular glutathione with buthionine sulphoximine (0.001 mM) and at concentrations of CHMA up to 3.0 mg per plate eliciting cytotoxicity. Glutathione depletion did however potentiate cytotoxicity and hepatocyte glutathione concentrations were lowered by CHMA treatment indicating reactivity of CHMA in the cell.

引用

页码：57 / 63

页数：7

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