THE FRACTIONATION OF HUMAN PLASMA-PROTEINS .1. AFFINITY PURIFICATION OF HUMAN-COMPLEMENT PROPERDIN

被引：5

作者：

DISCIPIO, RG

机构：

[1] Scripps Clin, Res Inst, Dept Immunol Imm 18, La Jolla, CA 92037

来源：

PROTEIN EXPRESSION AND PURIFICATION | 1994年 / 5卷 / 02期

关键词：

D O I：

10.1006/prep.1994.1026

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method was developed for the affinity purification of human complement properdin. The preparation is part of an integrated scheme in which over 20 human plasma proteins can be recovered in a highly purified form. The yield of properdin was 5.9 mg from 3 liters of plasma, amounting to a 28% recovery. The crucial step in the purification was the application of affinity chromatography using C3b-ester-Sepharose. (C) 1994 Academic Press,Inc.

引用

页码：164 / 169

页数：6

共 28 条

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