HUMAN BRAIN NATRIURETIC PEPTIDE-LIKE IMMUNOREACTIVITY IN HUMAN BRAIN

被引：84

作者：

TAKAHASHI, K ^{[1
]}

TOTSUNE, K ^{[1
]}

SONE, M ^{[1
]}

OHNEDA, M ^{[1
]}

MURAKAMI, O ^{[1
]}

ITOI, K ^{[1
]}

MOURI, T ^{[1
]}

机构：

[1] TOHOKU UNIV,SCH MED,DEPT PATHOL 2,AOBA KU,SENDAI,MIYAGI 980,JAPAN

来源：

PEPTIDES | 1992年 / 13卷 / 01期

关键词：

BRAIN NATRIURETIC PEPTIDE; ATRIAL NATRIURETIC PEPTIDE; RADIOIMMUNOASSAY; CHROMATOGRAPHY; HUMAN BRAIN;

D O I：

10.1016/0196-9781(92)90149-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The presence of immunoreactive human brain natriuretic peptide in the human brain was studied with a specific radioimmunoassay for human brain natriuretic peptide-32. This assay showed no significant cross-reaction with human alpha atrial natriuretic peptide, porcine brain natriuretic peptide or rat brain natriuretic peptide. Immunoreactive human brain natriuretic peptide was found in all 5 regions of human brain examined (cerebral cortex, thalamus, cerebellum, pons and hypothalamus) (0.6-6.7 pmol/g wet weight, n = 3). These values were comparable to the concentrations of immunoreactive alpha atrial natriuretic peptide in human brain (0.5-10.1 pmol/g wet weight). However, Sephadex G-50 column chromatography showed that the immunoreactive human brain natriuretic peptide in the human brain eluted earlier than synthetic human brain natriuretic peptide-32. These findings suggest that human brain natriuretic peptide is present in the human brain mainly as larger molecular weight forms.

引用

页码：121 / 123

页数：3

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