THE ALLIUM TEST IN WASTE-WATER MONITORING

被引:118
作者
FISKESJO, G
机构
[1] Department of Genetics, University of Lund, Lund
来源
ENVIRONMENTAL TOXICOLOGY AND WATER QUALITY | 1993年 / 8卷 / 03期
关键词
D O I
10.1002/tox.2530080306
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
The common onion Allium cepa L. (2n = 16) makes a very convenient test system for estimating harmful effects of chemicals on biological materials. Because of its excellent chromosome conditions, the Allium material has been widely exploited for such purposes since it was first introduced as a test system by A. Levar in 1938. The Allium test has later been extended to monitoring environmental risk factors as well. When wastewater from a chemical factory in southern Sweden caused severe pollution of an adjacent river, the growth restriction on Allium roots was taken as a measure of the degree of pollution in the river, and the result of the Allium test was admitted as evidence in a lawsuit against the factory. The Allium test has shown good correlation with other test systems, involving general toxicity (root growth) and genotoxicity (chromosome aberrations). Living material of Allium is easily stored and handled. It is inexpensive and assures very clear chromosomes. An Allium test takes a relatively short time to carry through: 2 days for chromosome preparations and 3-4 days for measurement of the root growth. From the growth curves, EC (effect concentration) values are obtained. (C) 1993 John Wiley & Sons, Inc.
引用
收藏
页码:291 / 298
页数:8
相关论文
共 17 条
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