Purification and Characterization of Bovine Serum Albumin Using Chromatographic Method

被引：13

作者：

Balkani, Sanaz ^{[1
,2
]}

Shamekhi, Sara ^{[1
]}

Raoufinia, Ramin ^{[1
]}

Parvan, Reza ^{[1
]}

Abdolalizadeh, Jalal ^{[3
,4
]}

机构：

[1] Tabriz Univ Med Sci, Drug Appl Res Ctr, Tabriz, Iran

[2] Univ Tehran, Kish Int Campus, Tehran, Iran

[3] Tabriz Univ Med Sci, Paramed Fac, Tabriz, Iran

[4] Tabriz Univ Med Sci, Immunol Res Ctr, Tabriz, Iran

来源：

ADVANCED PHARMACEUTICAL BULLETIN | 2016年 / 6卷 / 04期

关键词：

Bovine serum albumin (BSA); Chromatography; Immunoaffinity purification; Polyclonal antibody; Western blotting;

D O I：

10.15171/apb.2016.080

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Purpose: Albumin is an abundant protein of blood and has many biopharmaceutical applications. The aim of this study was to purify bovine serum albumin (BSA) using produced rabbit anti-BSA antibody. Methods: The polyclonal antibody was produced against the BSA in rabbits. Then, the pure BSA was injected to three white New Zealand rabbits. ELISA test was done to evaluate antibody production. After antibody purification, the purified antibody was attached to CNBr-activated sepharose and finally it was used for purification of albumin from bovine serum. Western blotting analysis was used for functional assessment of immunoaffinity purified BSA. Results: The titer of anti-bovine albumin determined by ELISA was obtained 1: 256000. The SDS-PAGE showed up to 98% purity of isolated BSA and western blotting confirmed the BSA functionality. Purified bovine serum albumin by affinity chromatography showed a single band with molecular weight of 66 KDa. Conclusion: Affinity chromatography using produced rabbit anti-BSA antibody would be an economical and safe method for purification of BSA.

引用

页码：651 / 654

页数：4

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