NUCLEOTIDE-METABOLISM BY MICROSOMAL UDP-GLUCURONYLTRANSFERASE AND NUCLEOSIDE DIPHOSPHATASE AS DETERMINED BY P-31 NMR-SPECTROSCOPY

被引:19
作者
FINCH, SAE [1 ]
SLATER, TF [1 ]
STIER, A [1 ]
机构
[1] BRUNEL UNIV,DEPT BIOCHEM,UXBRIDGE UX8 3PH,ENGLAND
关键词
D O I
10.1042/bj1770925
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
31P n.m.r. spectroscopy was used to study the nucleotide kinetics of UDP-glucuronyltransferase and associated reactions in the liver microsomal fraction. The effects of Mg2+ and EDTA on these reactions were investigated qualitatively. It was found that the rabbit microsomal fraction has no nucleoside pyrophosphatase activity, that UDP was immediately hydrolysed and that it was released from the microsomal surface. Reverse glucuronyltransferase could be demonstrated. The results are discussed with reference to functional coupling of UDP-glucuronyltransferase to other enzymes and the effects of Mg2+ and EDTA on the system.
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页码:925 / 930
页数:6
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