DEVELOPMENT OF RADIOIMMUNOASSAY FOR TG-51, A NEW ANTIULCER DRUG, AND ITS APPLICATION

被引：0

作者：

TAKAHAMA, S ^{[1
]}

OHBA, T ^{[1
]}

NARUCHI, T ^{[1
]}

YAMAMOTO, I ^{[1
]}

机构：

[1] OKAYAMA UNIV,FAC PHARMACEUT SCI,DEPT IMMUNOCHEM,OKAYAMA 700,JAPAN

来源：

JOURNAL OF IMMUNOASSAY | 1992年 / 13卷 / 02期

关键词：

RADIOIMMUNOASSAY; TG-51; ANTIULCER DRUG; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; PLASMA LEVELS OF TG-51;

D O I：

10.1080/15321819208021225

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive and specific radioimmunoassay for a new anti-ulcer drug TG-51 has been developed and applied to the evaluation of its Pharmacokinetics in humans. The antiserum was raised in rabbits against an immunogen of N-Acetyl-TG-51 coupled to human serum albumin. The radioactive compound was prepared by acetylating TG-51 with 3H-Acetic anhydride. The separation of free and antibody-bound N-Acetyl-TG-51 was performed by the dextran coated charcoal technique. TG-51 in biological fluids could be quantitatively converted to N-Acetyl-TG-51 prior to radioimmunoassay. This assay system made it possible to ascertain values of 3 ng/ml of TG-51 in plasma using 100-mu-1 of samples. Results were in good agreement with a high performance liquid chromatography method (HPLC), and the detection limit was raised 25 fold. The accuracy and reproducibility were also satisfactory. By use of this assay method, plasma levels of TG-51 could be determined after a single oral administration of clinical doses of human volunteers.

引用

页码：163 / 180

页数：18

共 10 条

[1] HOSHINA K, 1986, DIGEST DIS SCI, V31, pS348
[2] HOSHINA K, 1985, ARZNEIMITTEL-FORSCH, V35, P439
[3] TANABE H, 1986, DIGEST DIS SCI, V31, pS200
[4] TANABE H, 1988, XENOBIO METABOL DISP, V3, P3
[5] TANABE H, 1988, XENOBIO METABOL DISP, V3, P25
[6] YAMAMOTO I, 1982, IMMUNOPHARMACOLOGY, V4, P331, DOI 10.1016/0162-3109(82)90054-6
[7] DEVELOPMENT OF ENZYME-IMMUNOASSAY FOR PLATONIN (NK 19)
YAMAMOTO, I
MORISHITA, K
[J]. JOURNAL OF IMMUNOASSAY, 1986, 7 (1-2): : 17 - 35
[8] ENZYME-IMMUNOASSAY OF CYCLIC ADENOSINE-3',5'-MONOPHOSPHATE (AMP) USING BETA-D-GALACTOSIDASE AS LABEL
YAMAMOTO, I
TSUJI, J
[J]. IMMUNOPHARMACOLOGY, 1981, 3 (01): : 53 - 59
[9] YAMAMOTO I, 1982, Journal of Immunoassay, V3, P155, DOI 10.1080/15321818208056993
[10] YAMAZAKI N, 1987, JPN J PHARMACOL, V43, pP232

← 1 →