ALCOHOL-DEHYDROGENASE AS AN ASSAY FOR BIOLOGICAL ZINC IN MAIZE - EVIDENCE FOR A ZINC AVAILABILITY PEPTIDE (ZAP)

被引：0
|
作者：
PRYOR, A
机构：
来源：
MAYDICA | 1991年 / 36卷 / 02期
关键词：
ZEA-MAYS L; ALCOHOL DEHYDROGENASE; ZINC; ZINC METABOLISM;
D O I：
暂无
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
The zinc dependent reactivation of partially purified alcohol dehydrogenase subunits provided the basis of an assay capable of measuring Zn++ at concentrations of 1-mu-M (0.065 ppm) or lower. Pure ADH subunits responded poorly to Zn++ but could be reactivated by a fraction called. Zinc Availability Peptide (ZAP), isolated in a 30000 x g supermatant fraction from excised embryos. ZAP activity was protease sensitive and relatively heat stable. A non-specific protein, BSA, did not substitute for ZAP activity.
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页码：135 / 140
页数：6
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