TRANSCRIPTIONAL ANALYSIS OF THE GLNB GLNA REGION OF RHIZOBIUM-LEGUMINOSARUM BIOVAR VICIAE

被引：34

作者：

CHIURAZZI, M ^{[1
]}

IACCARINO, M ^{[1
]}

机构：

[1] CNR,IST INT GENET & BIOFIS,VIA MARCONI 10,I-80125 NAPLES,ITALY

来源：

MOLECULAR MICROBIOLOGY | 1990年 / 4卷 / 10期

关键词：

D O I：

10.1111/j.1365-2958.1990.tb00550.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report that the glnB and gln A genes of Rhizobium leguminosarum biovar viciae are preceded by promoters located upstream of each gene. We find the presence of a glnB–glnA and a glnA mRNA whose intracellular concentration changes two‐ to three‐fold when R leguminosarum is grown on different nitrogen sources. Primer extension analysis shows unique transcriptional initiation sites upstream of glnB and glnA. The glnB promoter is rpoN(ntrA)‐dependent, while the glnA promoter does not contain a typical consensus sequence for previously described promoters. In Klebsiella pneumoniae the glnB promoter requires active ntrC and ntrA genes and a DNA fragment containing 53 nucleotides upstream of the transcription initiation site shows full promoter activity, thus indicating that no NtrC binding sites are necessary for this activation in the glnB upstream region. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：1727 / 1735

页数：9

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