COMPARATIVE BIOCHEMICAL-ANALYSIS OF PURIFIED S-ANTIGEN FROM HUMAN, BOVINE, PORCINE AND RAT RETINA

被引：17

作者：

BANGA, JP ^{[1
]}

KASP, E ^{[1
]}

SULEYMAN, S ^{[1
]}

BROWN, E ^{[1
]}

ELLIS, BA ^{[1
]}

SANDERS, MD ^{[1
]}

DUMONDE, DC ^{[1
]}

机构：

[1] UNITED MED & DENT SCH GUYS & ST THOMAS HOSP, DEPT IMMUNOL & MED OPHTHALMOL, ST THOMAS CAMPUS, LONDON SE1 7EH, ENGLAND

来源：

EXPERIMENTAL EYE RESEARCH | 1987年 / 44卷 / 02期

关键词：

D O I：

10.1016/S0014-4835(87)80004-0

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

Highly purified S-antigen from human, bovine, porcine and rat retina was used to ascertain the molecular weights, isoelectric points, amino-acid composition and presence of carbohydrate moieties. All S-antigen preparations comigrate to 50,000 MW on SDS-polyacrylamide gels and demonstrate microheterogeneity upon isoelectric focusing. Human S-antigen exhibits two bands at ph 5.9 and 5.6, whilst with bovine S-antigen a broad band at pH 5.9 was observed. Porcine and rat S-antigen gave four bands, focusing between pH 5.0-5.6. Two-dimensional gel analysis revealed that all the four focused porcine polypeptides migrate to 50,000 MW. Western-blot analysis following isoelectric focusing of human and porcine S-antigen showed that all polypeptide bands react specifically with polyclonal and monoclonal antibodies to S-antigen. The reasons for the apparent heterogeneity of S-antigen by isolectric focusing are discussed. All S-antigen preparations were weakly positive for periodic acid Schiff staining, and specifically bound radiolabelled Lens culinaris lectin on Western-blot analysis. These results confirm the glycoprotein nature of S-antigen which may explain the finding of multiple bands found on isolectric focusing. Amino-acid analysis reveals the high percentage of non-polar amino acids and may explain the apparent hydrophobic behaviour of the isolated protein.

引用

页码：199 / 207

页数：9

共 24 条

[1] A COMMON EPITOPE IDENTIFIED BY A MONOCLONAL-ANTIBODY, MID-2, PRESENT ON ALL LEUKOCYTES AND ASSOCIATED WITH A GROUP OF HIGH MOLECULAR-WEIGHT GLYCOPEPTIDES [J].