ANALYSIS OF THIAMINE AND ITS PHOSPHATE-ESTERS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：69

作者：

ISHII, K

SARAI, K

SANEMORI, H

KAWASAKI, T

机构：

[1] HIROSHIMA UNIV,SCH MED,DEPT NEUROPSYCHIAT,HIROSHIMA 734,JAPAN

[2] HIROSHIMA UNIV,SCH MED,DEPT BIOCHEM,HIROSHIMA 734,JAPAN

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 97卷 / 01期

关键词：

D O I：

10.1016/0003-2697(79)90345-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

High-performance liquid chromatography was used to separate thiamine and its phosphate esters after conversion to corresponding highly fluorescent thiochrome derivatives by alkaline oxidation. These compounds were absorbed on LiChrosorb-NH2, eluted with acetonitrile-90 mm potassium phosphate buffer (pH 8.4), and determined spectrofluorometrically. A complete, rapid, and quantitative separation of thiochrome and its phosphate derivatives was made and the minimum amount detected was 1 pmol for each of these compounds. © 1979.

引用

页码：191 / 195

页数：5

共 5 条

[1]

HAMANAKA W, 1965, VITAMINS, V32, P576

[2]

ITOKAWA Y., 1970, Methods in Enzymology, V18A, P91, DOI 10.1016/0076-6879(71)18284-5

[3] QUANTITATIVE SEPARATION OF TRIPHOSPHOTHIAMINE IN BIOLOGICAL MATERIALS AND ITS FORMATION FROM S35-THIAMINE IN RAT LIVER [J].