ORGANIZATION OF NUCLEOLAR DNA IN RESTING LYMPHOCYTES AS REVEALED BY THE DIAMINOBENZIDINE TECHNIQUE

被引：14

作者：

ANTEUNIS, A

POUCHELET, M

GANSMULLER, A

ROBINEAUX, R

机构：

[1] Centre de Physiologie et d'Immunologie Cellulaires, INSERM U.104, CNRS, et Association Cl. Bernard, Hôpital Saint-Antoine

来源：

JOURNAL OF ULTRASTRUCTURE RESEARCH | 1979年 / 69卷 / 01期

关键词：

D O I：

10.1016/S0022-5320(79)80039-8

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Nucleolar DNA organization in resting lymphocytes was studied with oxidized diaminobenzidine, which is a highly selective stain for nucleic acids at low pH. This technique, combined with enzymatic digestions, enables us to observe a gradual decondensation of a portion of the perinucleolar DNA until it becomes spread into the fibrillar RNP zone of the nucleolus. The presence and significance of these dispersed DNA structures within this fibrillar zone are discussed. It is concluded that the gradual decondensation of certain portions of the perinucleolar chromatin is a general feature of nucleolar organization. © 1979 Academic Press, Inc.

引用

页码：22 / 27

页数：6

共 26 条

[1]

ANTEUNIS A, 1977, BIOL CELLULAIRE, V29, pA4

[2]

ANTEUNIS A, 1975, CR ACAD SCI D NAT, V281, P901

[3]

ANTEUNIS A, 1973, CR ACAD SCI D NAT, V277, P1169

[4]

ANTEUNIS AH, 1976, J CELL BIOL, V70, pA396

[5] NUCLEAR PROTEIN MATRIX - ASSOCIATION WITH NEWLY SYNTHESIZED DNA [J].

BEREZNEY, R ;

COFFEY, DS .

SCIENCE, 1975, 189 (4199) :291-293

[6]

GEYER G, 1977, HDB HISTOCHEMIE 3, V1, P204

[7]

GOESSENS G, 1974, CR ACAD SCI D NAT, V279, P991

[8]

GOESSENS G, 1973, CR ACAD SCI D NAT, V277, P325

[9] HIGH-RESOLUTION AUTORADIOGRAPHIC STUDIES OF EHRLICH TUMOR-CELL NUCLEOLI - NUCLEOLAR LABELING AFTER [ACTINOMYCIN-H-3] D BINDING TO DNA OR AFTER [TDR-H-3] OR [H-3]URIDINE INCORPORATION IN NUCLEIC-ACIDS [J].

GOESSENS, G .

EXPERIMENTAL CELL RESEARCH, 1976, 100 (01) :88-94

[10]

GOESSENS G, 1974, J MICROSC-PARIS, V19, pA12

← 1 2 3 →