ISOLATION AND IDENTIFICATION OF 6 PNEUMOCYSTIS-CARINII GENES UTILIZING CODON BIAS

被引：25
作者：
FLETCHER, LD
BERGER, LC
PEEL, SA
BARIC, RS
TIDWELL, RR
DYKSTRA, CC
机构：
[1] UNIV N CAROLINA,SCH MED,DEPT PATHOL,CB 7525,811 BRINKHOUS BULLITT,CHAPEL HILL,NC 27599
[2] UNIV N CAROLINA,SCH PUBL HLTH,DEPT EPIDEMIOL,CHAPEL HILL,NC 27599
来源：
GENE | 1993年 / 129卷 / 02期
关键词：
NUCLEOTIDE SEQUENCE; CALMODULIN; BETA-TUBULIN; DNA POLYMERASE-II; RNA POLYMERASE-I; POLYMERASE-II AND POLYMERASE-III; AMINO ACID SEQUENCE HOMOLOGY; POLYMERASE CHAIN REACTION; AIDS;
D O I：
10.1016/0378-1119(93)90265-5
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Pneumocystis carinii pneumonia (PCP) is a leading cause of death among AIDS patients in the United States. Our analysis of P. carinii protein-coding genes has revealed a significant A + T codon bias. Polymerase chain reaction (PCR) was utilized to isolate and identify the genes encoding calmodulin, beta-tubulin, DNA polymerase II, and RNA polymerases I, II and III from P. carinii. Primer pairs were designed to incorporate P. carinii codon preference to known conserved protein regions from other organisms. This strategy should be useful for a large variety of P. carinii genes and assist in the comprehensive analysis of the genomic structure of this important pathogen.
引用
收藏
页码：167 / 174
页数：8
相关论文
共 43 条
[1]   EXTENSIVE HOMOLOGY AMONG THE LARGEST SUBUNITS OF EUKARYOTIC AND PROKARYOTIC RNA-POLYMERASES [J].
ALLISON, LA ;
MOYLE, M ;
SHALES, M ;
INGLES, CJ .
CELL, 1985, 42 (02) :599-610
[2]  
BABA ML, 1984, MOL BIOL EVOL, V1, P442
[3]   A CONSERVED 3'-]5' EXONUCLEASE ACTIVE-SITE IN PROKARYOTIC AND EUKARYOTIC DNA-POLYMERASES [J].
BERNAD, A ;
BLANCO, L ;
LAZARO, JM ;
MARTIN, G ;
SALAS, M .
CELL, 1989, 59 (01) :219-228
[4]  
BERSHADSKY AD, 1989, CELLULAR ORGANELLES, P79
[5]   NUCLEAR PRE-MESSENGER-RNA INTRONS - ANALYSIS AND COMPARISON OF INTRON SEQUENCES FROM TETRAHYMENA-THERMOPHILA AND OTHER EUKARYOTES [J].
CSANK, C ;
TAYLOR, FM ;
MARTINDALE, DW .
NUCLEIC ACIDS RESEARCH, 1990, 18 (17) :5133-5141
[6]  
DAVIS TN, 1987, METHOD ENZYMOL, V139, P248
[7]   A COMPARISON OF THE INTERACTION OF ANTHELMINTIC BENZIMIDAZOLES WITH TUBULIN ISOLATED FROM MAMMALIAN TISSUE AND THE PARASITIC NEMATODE ASCARIDIA-GALLI [J].
DAWSON, PJ ;
GUTTERIDGE, WE ;
GULL, K .
BIOCHEMICAL PHARMACOLOGY, 1984, 33 (07) :1069-1074
[8]   CLONING OF A BETA-TUBULIN GENE FROM PLASMODIUM-FALCIPARUM [J].
DELVES, CJ ;
RIDLEY, RG ;
GOMAN, M ;
HOLLOWAY, SP ;
HYDE, JE ;
SCAIFE, JG .
MOLECULAR MICROBIOLOGY, 1989, 3 (11) :1511-1519
[9]   A COMPREHENSIVE SET OF SEQUENCE-ANALYSIS PROGRAMS FOR THE VAX [J].
DEVEREUX, J ;
HAEBERLI, P ;
SMITHIES, O .
NUCLEIC ACIDS RESEARCH, 1984, 12 (01) :387-395
[10]   CLONING AND SEQUENCE OF A BETA-TUBULIN CDNA FROM PNEUMOCYSTIS-CARINII - POSSIBLE IMPLICATIONS FOR DRUG-THERAPY [J].
DYER, M ;
VOLPE, F ;
DELVES, CJ ;
SOMIA, N ;
BURNS, S ;
SCAIFE, JG .
MOLECULAR MICROBIOLOGY, 1992, 6 (08) :991-1001
12345