AMPLIFICATION OF JC VIRUS-DNA FROM BRAIN AND CEREBROSPINAL-FLUID OF PATIENTS WITH PROGRESSIVE MULTIFOCAL LEUKOENCEPHALOPATHY

被引：62
作者：
HENSON, J
ROSENBLUM, M
ARMSTRONG, D
FURNEAUX, H
机构：
[1] MEM SLOAN KETTERING CANC CTR,DEPT PATHOL,MOLEC NEUROONCOL LAB,1275 YORK AVE,NEW YORK,NY 10021
[2] MEM SLOAN KETTERING CANC CTR,DEPT MED,DIV INFECT DIS,NEW YORK,NY 10021
来源：
NEUROLOGY | 1991年 / 41卷 / 12期
关键词：
D O I：
10.1212/WNL.41.12.1967
中图分类号：
R74 [神经病学与精神病学];
学科分类号：
摘要：
We have established an assay to detect JC viral DNA in brain tissue and CSF of patients with progressive multifocal leukoencephalopathy (PML) and amplified a 106-base pair segment of the gene encoding JC viral large T antigen by polymerase chain reaction (PCR) from the brains of eight patients with PML and from one of six CSF samples. Brain and CSF of patients without PML did not yield JC virus-specific DNA amplification products. Southern analysis documented that the sequences of the 106-base pair PCR product and JC viral DNA are identical. This assay can be used in the diagnosis of PML and to investigate the biology of JC virus.
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页码：1967 / 1971
页数：5
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