EFFECT OF ACTIVATION-ENERGY MICROHETEROGENEITY ON 1ST-ORDER ENZYME DEACTIVATION

被引：17
作者：
MALHOTRA, A
SADANA, A
机构：
[1] Chemical Engineering Department, University of Mississippi, University Park, Mississippi
来源：
BIOTECHNOLOGY AND BIOENGINEERING | 1987年 / 30卷 / 01期
关键词：
BIOCHEMICAL ENGINEERING - CHEMICAL REACTIONS - Thermodynamics;
D O I：
10.1002/bit.260300116
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The influence of microheterogeneity on enzyme inactivation kinetics is examined. A continuous normal distribution of the thermal activation energy is assumed, and using this, a simple mathematical model is developed to find the activity–time trajectories for a microheterogeneous enzyme. Using an example, the model is used to show the quantitative effects of microheterogeneity such as increased order and stability observed during an enzyme inactivation. Experimental measurement of the extent of microheterogeneity in an enzyme sample is also discussed. Copyright © 1987 John Wiley & Sons, Inc.
引用
收藏
页码：108 / 116
页数：9
相关论文
共 10 条
[1]   HETEROGENEOUS DENATURATION OF ENZYMES - A DISTRIBUTED ACTIVATION-ENERGY MODEL WITH NONUNIFORM ACTIVITIES [J].
AGARWAL, PK .
BIOTECHNOLOGY AND BIOENGINEERING, 1985, 27 (11) :1554-1563
[2]   THE MICROHETEROGENEITY OF PROTEINS [J].
COLVIN, JR ;
SMITH, DB ;
COOK, WH .
CHEMICAL REVIEWS, 1954, 54 (04) :687-711
[3]  
DOI Y, 1983, J BIOL CHEM, V258, P5840
[4]  
FOSTER JF, 1965, J BIOL CHEM, V240, P2495
[5]  
FOSTER JF, 1968, CHEM SOC, V23, P25
[6]   THERMAL AND CHEMICAL DEACTIVATION OF SOLUBLE AND STABILIZED ENZYMES [J].
GRECO, G ;
MARRUCCI, G ;
GRIZZUTI, N ;
GIANFREDA, L .
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1984, 434 (DEC) :7-19
[7]   STABILITY OF IMMOBILIZED ALPHA-CHYMOTRYPSIN [J].
KAWAMURA, Y ;
NAKANISHI, K ;
MATSUNO, R ;
KAMIKUBO, T .
BIOTECHNOLOGY AND BIOENGINEERING, 1981, 23 (06) :1219-1236
[8]   ON THE THERMAL-DENATURATION OF GLUCOSE-OXIDASE [J].
MALIKKIDES, CO ;
WEILAND, RH .
BIOTECHNOLOGY AND BIOENGINEERING, 1982, 24 (08) :1911-1914
[9]   MULTIPLE FORMS OF ENZYMES - TISSUE, ONTOGENETIC, AND SPECIES SPECIFIC PATTERNS [J].
MARKERT, CL ;
MOLLER, F .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1959, 45 (05) :753-763
[10]   PROTEIN INTERACTIONS .36. DISTRIBUTION OF STABILITY IN A SYSTEM OF SERUM ALBUMIN MOLECULES [J].
STOKROVA, S ;
SPONAR, J .
COLLECTION OF CZECHOSLOVAK CHEMICAL COMMUNICATIONS, 1963, 28 (03) :659-&
1