C-MYC GENE ABNORMALITIES IN HIGH-GRADE AND CENTROBLASTIC-CENTROCYTIC NON-HODGKINS-LYMPHOMA

被引:2
作者
LEVASSEUR, M [1 ]
MIDDLETON, PG [1 ]
ANGUS, B [1 ]
PROCTOR, SJ [1 ]
NORDEN, J [1 ]
HOWARD, MR [1 ]
机构
[1] MED SCH NEWCASTLE UPON TYNE,LEUKAEMIA RES FUND LAB,NEWCASTLE TYNE NE2 4HH,TYNE & WEAR,ENGLAND
关键词
NON HODGKINS LYMPHOMA; C-MYC GENE;
D O I
10.3109/10428199509064933
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Fifty nine cases of high grade and centroblastic-centrocytic (cc) Non Hodgkins Lymphoma (NHL) were investigated for mutations and gross gene rearrangements in the 5' region of the c-MYC gene. Mutations in this region, and the presence of hypermutated c-MYC genes, have been associated with poor prognostic groups. All cases showed normal c-MYC gene organisation on Southern blot analysis indicating absence of gross gene rearrangements, PCR amplification and restriction digest analysis of the exon 1/intron 1 region revealed point mutations in 7 cases. No evidence for hypermutation was found. Mutations were relatively more common in high grade NHL (6/39) than in cc-NHL (1/20). There was no correlation with disease status at presentation or relapse or the presence of extranodal disease. The cc-NHL case with a c-MYC mutation subsequently transformed to high grade disease. These data suggest that hypermutation of the c-MYC gene is a relatively rare event in cc-NHL and high grade NHL and does not contribute to the aetiology in the majority of cases. Mutation of c-MYC in cc-NHL may predict transformation to high grade disease.
引用
收藏
页码:131 / 136
页数:6
相关论文
共 16 条
  • [1] Nacheva E., Dyer M.J.S., Fischer P., Stranks G., Heward J.M., Marcus R.E., Grace C., Karpas A., c-MYC translocations in de novo B-cell lineage acute leukaemias with t(14
  • [2] 18) (cell lines Karpas 231 and 353), Blood, 82, pp. 231-240, (1993)
  • [3] Ladanyi M., Offit K., Jhanwar S.C., Filippa D.A., Chaganti R.S.K., MYC rearrangement and translocations involving band 8q24 in diffuse large cell lymphomas, Blood, 77, pp. 1057-1063, (1991)
  • [4] Chaganti R.S.K., Doucette L.A., Offit K., Filippa D.A., Allen G.J., Condon M.R., Jhanwar S.C., Clarkson B.D., Leiberman P.H., Specific translocations in non-Hodgkins Lymphoma: Incidence, molecular detection, and histological and clinical correlations, Cancer cells 7: The molecular diagnostics of human cancer p33., (1989)
  • [5] Van Krieken J.H.J.M., Kluin P.H.M., The association of c-MYC rearrangements with specific types of human non-Hodgkins Lymphomas, Leukaemia and Lymphoma, 7, pp. 371-376, (1992)
  • [6] Rabbitts T.H., Hamlyn P.H., Baer R., Altered nucleotide sequences of a translocated c-MYC gene in Burkitt lymphoma, Nature, 306, pp. 760-765, (1983)
  • [7] Bradley J.F., Rothberg P.G., Ladanyi M., Chaganti R.S.K., Hypermutation of the MYC gene in diffuse large cell lymphomas with translocations involving band 8q24, Genes Chromosomes and Cancer, 7, pp. 128-130, (1993)
  • [8] Land H., Parada L.F., Weinberg R.A., Tumorigenic conversion of primary embryo fibroblasts requires at least two cooperating oncogenes, Nature, 304, pp. 596-602, (1983)
  • [9] Lefranc M.P., Rabbitts T.H., Two tandemly organized human genes encoding the T-cell gamma constant region show multiple rearrangements in different T-cell types, Nature, 316, pp. 464-466, (1985)
  • [10] Van Dongen J.J.M., Wolvers-Tettero I.L.M., Analysis of immunoglobulin and T-cell receptor genes, Clin. Chim. Acta, 198, pp. 1-174, (1991)