MUTATION CAUSING OVERPRODUCTION OF THE SHORT PEPTIDE COMPONENT-GAMMA OF PENICILLIN-BINDING PROTEIN-1BS OF ESCHERICHIA-COLI

被引：0

作者：

ISHINO, F ^{[1
]}

ITO, Y ^{[1
]}

UENO, S ^{[1
]}

TAMAKI, S ^{[1
]}

MATSUHASHI, M ^{[1
]}

机构：

[1] UNIV TOKYO, INST APPL MICROBIOL, 1-1-1 YAYOI, BUNKYO KU, TOKYO 113, JAPAN

来源：

JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY | 1990年 / 36卷 / 03期

关键词：

D O I：

10.2323/jgam.36.163

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An Escherichia coli mutant was isolated which overproduces component γ of penicillin-binding protein (PBP)-lBs. The mutant formed normal amounts of components α and its proteolytic product β. The mutation was located in mrcB, the structural gene coding for PBP-lBα and γ. The overproduction of the mutant-type PBP-lBγ was caused by an alteration of one nucleotide which changed the GGC coding for glycine at residue 8 from the N-terminal of PBP-lBγ to GAC coding for aspartate. © 1990, Applied Microbiology, Molecular and Cellular Biosciences Research Foundation. All rights reserved.

引用

页码：163 / 169

页数：7

共 12 条

[1] OPTIMAL CONDITIONS FOR MUTAGENESIS BY N-METHYL-N'-NITRO-N-NITROSOGUANIDINE IN ESCHERICHIA COLI K12
ADELBERG, EA
MANDEL, M
CHEN, GCC
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1965, 18 (5-6) : 788 - &
[2] LINKAGE MAP OF ESCHERICHIA-COLI K-12, EDITION-8
BACHMANN, BJ
[J]. MICROBIOLOGICAL REVIEWS, 1990, 54 (02) : 130 - 197
[3] THE NUCLEOTIDE-SEQUENCES OF THE PONA AND PONB GENES ENCODING PENICILLIN-BINDING PROTEINS 1A AND 1B OF ESCHERICHIA-COLI-K12
BROOMESMITH, JK
EDELMAN, A
YOUSIF, S
SPRATT, BG
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1985, 147 (02): : 437 - 446
[4] ISHINO F, 1988, P285
[5] OVERLAPPING OF THE CODING REGIONS FOR ALPHA-COMPONENT AND GAMMA-COMPONENT OF PENICILLIN-BINDING PROTEIN-1B IN ESCHERICHIA-COLI
KATO, J
SUZUKI, H
HIROTA, Y
[J]. MOLECULAR & GENERAL GENETICS, 1984, 196 (03): : 449 - 457
[6] NAKAGAWA J, 1984, J BIOL CHEM, V259, P3937
[7] MOLECULAR DIVERGENCE OF A MAJOR PEPTIDOGLYCAN SYNTHETASE WITH TRANSGLYCOSYLASE-TRANSPEPTIDASE ACTIVITIES IN ESCHERICHIA-COLI - PENICILLIN-BINDING PROTEIN 1BS
NAKAGAWA, J
MATSUHASHI, M
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1982, 105 (04) : 1546 - 1553
[8] PURIFIED PENICILLIN BINDING-PROTEINS 1BS FROM ESCHERICHIA-COLI MEMBRANE SHOWING ACTIVITIES OF BOTH PEPTIDOGLYCAN POLYMERASE AND PEPTIDOGLYCAN CROSSLINKING ENZYME
NAKAGAWA, J
TAMAKI, S
MATSUHASHI, M
[J]. AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1979, 43 (06): : 1379 - 1380
[9] SALSER W, 1977, COLD SPRING HARB SYM, V42, P985
[10] SPRATT BG, 1975, P NATL ACAD SCI USA, V72, P2990

← 1 2 →