THE USE OF WATER-SOLUBLE RADICAL SCAVENGERS TO DETECT HYDROXYL RADICAL FORMATION BY POLYMORPHONUCLEAR LEUKOCYTES

被引：5

作者：

THOMAS, MJ

SMITH, S

PANG, J

机构：

[1] Department of Biochemistry, Wake Forest University Medical Center, Bowman Gray School of Medicine, Winston-Salem

来源：

FREE RADICAL RESEARCH COMMUNICATIONS | 1991年 / 12-3卷

关键词：

RADICAL SCAVENGERS; HYDROXYL RADICAL; POLYMORPHONUCLEAR LEUKOCYTES; SUPEROXIDE; HYDROGEN PEROXIDE;

D O I：

10.3109/10715769109145767

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The polymorphonuclear leukocyte secretes both O2-and H2O2 when stimulated by various soluble or particulate stimuli. Since a rcaction involving iron, O2-, and H2O2 could generate the hydroxyl radical (HO.) there has been speculation that the HO-may participate in the bactericidal activity of the neutroph-il. A variety of water-soluble HO. scavengers have been used to test for the participation of HO. and the results imply that HO. might participate. However, other workers have not been able to detect the formation of significant amounts of HO-by the activated neutrophil. We have examined the effect of several commonly used HO. radical scavengers on the ability of the neutrophil to secrete O2-and H2O2. Several of these compounds actively inhibit secretion without affecting the viability of the neutrophil. After considering the various complications inherent in using water soluble radical scavengers, we suggest that they only be used with well defined experimental systems. © 1991 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：53 / 57

页数：5

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