PRESENCE OF HERPES-SIMPLEX VIRUS TYPE-2 DNA IN HAMSTER-CELLS ONCOLOGICALLY TRANSFORMED BY ULTRAVIOLET-INACTIVATED VIRUS

被引：7
作者：
BIBORHARDY, V
KESSOUS, A
SIMARD, R
机构：
来源：
CANADIAN JOURNAL OF BIOCHEMISTRY | 1979年 / 57卷 / 06期
关键词：
D O I：
10.1139/o79-106
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Herpes simplex virus type 2 (HSV-2) DNA has been detected by molecular hybridization in hamster fibroblast cells oncogenically transformed by ultraviolet-irradiation virus. At early passages after cloning in soft agar, about 40% of the HSV-2 genome was present in all the transformed cell lines at one to six copies per cell. In cell lines derived from tumors induced by these cells, the same percentage of the HSV-2 genome was also found with more uniform number of copies (between two and three). Thus the presence of viral DNA seems to be necessary for the maintenance of the transformed state in these cell lines.
引用
收藏
页码：867 / 872
页数：6
相关论文
共 31 条
[11]   HERPES-SIMPLEX VIRUS-DNA IN TRANSFORMED-CELLS - SEQUENCE COMPLEXITY IN 5 HAMSTER CELL LINES AND ONE DERIVED HAMSTER TUMOR [J].
FRENKEL, N ;
LOCKER, H ;
COX, B ;
ROIZMAN, B ;
RAPP, F .
JOURNAL OF VIROLOGY, 1976, 18 (03) :885-893
[12]   DNA FRAGMENT OF HERPES-SIMPLEX 2 AND ITS TRANSCRIPTION IN HUMAN CERVICAL-CANCER TISSUE [J].
FRENKEL, N ;
ROIZMAN, B ;
CASSAI, E ;
NAHMIAS, A .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1972, 69 (12) :3784-3789
[13]  
JONES KW, 1978, ONCOGENESIS HERPESVI, V3
[14]   SIZE, COMPOSITION, AND STRUCTURE OF DEOXYRIBONUCLEIC ACID OF HERPES SIMPLEX VIRUS SUBTYPES 1 AND 2 [J].
KIEFF, ED ;
BACHENHEIMER, SL ;
ROIZMAN, B .
JOURNAL OF VIROLOGY, 1971, 8 (02) :125-+
[15]   PRESENCE OF A HERPES-SIMPLEX VIRUS DNA FRAGMENT IN AN L CELL CLONE OBTAINED AFTER INFECTION WITH IRRADIATED HERPES-SIMPLEX VIRUS-1 [J].
KRAISELBURD, E ;
GAGE, LP ;
WEISSBACH, A .
JOURNAL OF MOLECULAR BIOLOGY, 1975, 97 (04) :533-542
[16]   AMPLIFICATION AND CHARACTERIZATION OF A BETA-GLOBIN GENE SYNTHESIZED INVITRO [J].
MANIATIS, T ;
KEE, SG ;
EFSTRATIADIS, A ;
KAFATOS, FC .
CELL, 1976, 8 (02) :163-182
[17]   PROCEDURE FOR ISOLATION OF DEOXYRIBONUCLEIC ACID FROM MICRO-ORGANISMS [J].
MARMUR, J .
JOURNAL OF MOLECULAR BIOLOGY, 1961, 3 (02) :208-&
[18]   THERMAL ELUTION CHROMATOGRAPHY AND RESOLUTION OF NUCLEIC-ACIDS ON HYDROXYLAPATITE [J].
MARTINSON, HG ;
WAGENAAR, EB .
ANALYTICAL BIOCHEMISTRY, 1974, 61 (01) :144-154
[19]   INTRODUCTION OF HERPES-SIMPLEX VIRUS THYMIDINE KINASE GENE INTO MOUSE CELLS USING VIRUS-DNA OR TRANSFORMED-CELL DNA [J].
MINSON, AC ;
WILDY, P ;
BUCHAN, A ;
DARBY, G .
CELL, 1978, 13 (03) :581-587
[20]   DETECTION OF VIRUS DNA SEQUENCES IN A HERPES TYPE-2 TRANSFORMED HAMSTER CELL LINE (333-8-9) [J].
MINSON, AC ;
THOULESS, ME ;
EGLIN, RP ;
DARBY, G .
INTERNATIONAL JOURNAL OF CANCER, 1976, 17 (04) :493-500
1234