PRESENCE OF HERPES-SIMPLEX VIRUS TYPE-2 DNA IN HAMSTER-CELLS ONCOLOGICALLY TRANSFORMED BY ULTRAVIOLET-INACTIVATED VIRUS

被引：7
作者：
BIBORHARDY, V
KESSOUS, A
SIMARD, R
机构：
来源：
CANADIAN JOURNAL OF BIOCHEMISTRY | 1979年 / 57卷 / 06期
关键词：
D O I：
10.1139/o79-106
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Herpes simplex virus type 2 (HSV-2) DNA has been detected by molecular hybridization in hamster fibroblast cells oncogenically transformed by ultraviolet-irradiation virus. At early passages after cloning in soft agar, about 40% of the HSV-2 genome was present in all the transformed cell lines at one to six copies per cell. In cell lines derived from tumors induced by these cells, the same percentage of the HSV-2 genome was also found with more uniform number of copies (between two and three). Thus the presence of viral DNA seems to be necessary for the maintenance of the transformed state in these cell lines.
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页码：867 / 872
页数：6
相关论文
共 31 条
[1]   TRANSFER OF GENE FOR THYMIDINE KINASE TO THYMIDINE KINASE-DEFICIENT HUMAN CELLS BY PURIFIED HERPES-SIMPLEX VIRAL-DNA [J].
BACCHETTI, S ;
GRAHAM, FL .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (04) :1590-1594
[2]  
Britten R J, 1974, Methods Enzymol, V29, P363
[3]   REPEATED SEQUENCES IN DNA [J].
BRITTEN, RJ ;
KOHNE, DE .
SCIENCE, 1968, 161 (3841) :529-&
[4]  
BRITTEN RJ, 1970, CARNEGIE I WASH YEAR, V68, P378
[5]   CELLS TRANSFORMED BY HUMAN HERPESVIRUS TYPE 2 TRANSCRIBE VIRUS-SPECIFIC RNA SEQUENCES SHARED BY HERPESVIRUS TYPES 1 AND 2 [J].
COLLARD, W ;
THORNTON, H ;
GREEN, M .
NATURE-NEW BIOLOGY, 1973, 243 (130) :264-266
[6]   CLONAL DERIVATIVES OF A HERPES TYPE-2 TRANSFORMED HAMSTER CELL LINE (333-8-9) - CYTOGENETIC ANALYSIS, TUMORIGENICITY AND VIRUS SEQUENCE DETECTION [J].
COPPLE, CD ;
MCDOUGALL, JK .
INTERNATIONAL JOURNAL OF CANCER, 1976, 17 (04) :501-510
[7]   HERPES-SIMPLEX VIRUS AS A SOURCE F THYMIDINE KINASE FOR THYMIDINE KINASE-DEFICENT MOUSE CELLS - SUPPRESSION AND REACTIVATION OF VIRAL ENZYME [J].
DAVIDSON, RL ;
ADELSTEIN, SJ ;
OXMAN, MN .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1973, 70 (07) :1912-1916
[8]   QUANTITATION OF VIRAL DNA PRESENT IN CELLS TRANSFORMED BY UV-IRRADIATED HERPES-SIMPLEX VIRUS [J].
DAVIS, DB ;
KINGSBURY, DT .
JOURNAL OF VIROLOGY, 1976, 17 (03) :788-793
[9]   ONCOGENIC TRANSFORMATION OF HAMSTER EMBRYO CELLS AFTER EXPOSURE TO INACTIVATED HERPES-SIMPLEX VIRUS TYPE-1 [J].
DUFF, R ;
RAPP, F .
JOURNAL OF VIROLOGY, 1973, 12 (02) :209-217
[10]   PROPERTIES OF HAMSTER EMBRYO FIBROBLASTS TRANSFORMED IN-VITRO AFTER EXPOSURE TO ULTRAVIOLET-IRRADIATED HERPES SIMPLEX VIRUS TYPE 2 [J].
DUFF, R ;
RAPP, F .
JOURNAL OF VIROLOGY, 1971, 8 (04) :469-+
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