SEPARATION OF 5BETA-CHOLESTANE-3ALPHA,7ALPHA,12ALPHA,26-TETROL OXIDOREDUCTASE ETHANOL-NAD OXIDOREDUCTASE 5BETA-CHOLESTANE-3ALPHA,7ALPHA,12ALPHA-TRIOL-26-AL OXIDOREDUCTASE AND ACETALDEHYDE-NAD OXIDOREDUCTASE FROM SOLUBLE FRACTION OF RAT LIVER BY GEL FILTRATION

被引：18

作者：

OKUDA, K

TAKIGAWA, N

机构：

[1] Department of Biochemistry, Hiroshima University School of Dentistry, Hiroshima

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1968年 / 33卷 / 05期

关键词：

D O I：

10.1016/0006-291X(68)90229-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the previous report (Okuda et al., 1968) it was suggested that TeHC, which is an intermediate in the conversion of cholesterol to cholic acid (Danielsson, 1963), is dehydrogenated to THCA by two NAD-linked enzymes, namely TeHC-NAD oxidoreductase and THAL-NAD oxidoreductase in rat liver soluble fraction. In this communication, it will be reported that the TeHC-NAD oxidoreductase and THAL-NAD oxidoreductase from the soluble fraction of rat liver are distinct and separate enzymes, furthermore it will be suggested that TeHC-NAD oxidoreductase activity and ethanol-NAD oxidoreductase (EC 1.1.1.1) activity are due to the same enzyme, and THAL-NAD oxidoreductase activity and acetaldehyde-NAD oxidoreductase (EC 1.2.1.3) activity are due to the same enzyme. © 1968.

引用

页码：788 / &

共 7 条

[1]

COLOWICK SP, 1955, METHODS ENZYMOLOG ED, V1, P514

[2] THE ASSAY AND SPECIFIC ACTIVITY OF CRYSTALLINE ALCOHOL DEHYDROGENASE OF HORSE LIVER [J].