SECONDARY STRUCTURE OF THE PARTICLE ASSOCIATING DOMAIN OF APOLIPOPROTEIN-B-100 IN LOW-DENSITY-LIPOPROTEIN BY ATTENUATED TOTAL-REFLECTION INFRARED-SPECTROSCOPY

被引：47

作者：

GOORMAGHTIGH, E ^{[1
]}

CABIAUX, V ^{[1
]}

DEMEUTTER, J ^{[1
]}

ROSSENEU, M ^{[1
]}

RUYSSCHAERT, JM ^{[1
]}

机构：

[1] ST JAN HOSP,BRUGGE,BELGIUM

来源：

BIOCHEMISTRY | 1993年 / 32卷 / 23期

关键词：

D O I：

10.1021/bi00074a023

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The secondary structure of the human low-density lipoprotein (LDL) apo B-100 fragment embedded in the lipid domain of the particle has been investigated by Fourier transform attenuated total reflection infrared spectroscopy (FTIR-ATR). The solvent-exposed region of the protein was hydrolyzed by using different proteases (alpha-chymotrypsin, trypsin, proteinase K) for incubation times varying between 24 min and 48 h. Analysis of the FTIR-ATR spectra after repurification of the digested LDL particle indicates the same trend for all the hydrolysis conditions tested: the peptides remaining associated with the particle are rich in beta-sheet structure. Dichroism spectra reveal that at least part of the beta-sheets is associated with the phospholipid component of the particle.

引用

页码：6104 / 6110

页数：7

共 13 条

[1] THE BINDING OF VERY-LOW-DENSITY LIPOPROTEIN REMNANTS TO THE LOW-DENSITY-LIPOPROTEIN RECEPTOR IN FAMILIAL DEFECTIVE APOLIPOPROTEIN-B-100
MAHER, VMG
GALLAGHER, JJ
MYANT, NB
ATHEROSCLEROSIS, 1993, 102 (01) : 51 - 61
[2] EVALUATION OF THE SECONDARY STRUCTURE OF APO B-100 IN LOW-DENSITY LIPOPROTEIN (LDL) BY INFRARED-SPECTROSCOPY
GOORMAGHTIGH, E
DEMEUTTER, J
VANLOO, B
BRASSEUR, R
ROSSENEU, M
RUYSSCHAERT, JM
BIOCHIMICA ET BIOPHYSICA ACTA, 1989, 1006 (01) : 147 - 150
[3] STRUCTURE OF THE APOLIPOPROTEIN A-IV/LIPID DISCOIDAL COMPLEXES - AN ATTENUATED TOTAL-REFLECTION POLARIZED FOURIER-TRANSFORM INFRARED-SPECTROSCOPY STUDY
LINS, L
BRASSEUR, R
ROSSENEU, M
VANLOO, B
RUYSSCHAERT, JM
BIOCHIMICA ET BIOPHYSICA ACTA, 1993, 1149 (02) : 267 - 277
[4] SECONDARY STRUCTURE OF THE MEMBRANE-BOUND FORM OF THE PORE-FORMING DOMAIN OF COLICIN-A - AN ATTENUATED TOTAL-REFLECTION POLARIZED FOURIER-TRANSFORM INFRARED-SPECTROSCOPY STUDY
GOORMAGHTIGH, E
VIGNERON, L
KNIBIEHLER, M
LAZDUNSKI, C
RUYSSCHAERT, JM
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1991, 202 (03): : 1299 - 1305
[5] MONOCLONAL-ANTIBODIES TO HUMAN LOW-DENSITY-LIPOPROTEIN IDENTIFY DISTINCT AREAS ON APOLIPOPROTEIN-B-100 RELEVANT TO THE LOW-DENSITY LIPOPROTEIN-RECEPTOR INTERACTION
FANTAPPIE, S
CORSINI, A
SIDOLI, A
UBOLDI, P
GRANATA, A
ZANELLI, T
ROSSI, P
MARCOVINA, S
FUMAGALLI, R
CATAPANO, AL
JOURNAL OF LIPID RESEARCH, 1992, 33 (08) : 1111 - 1121
[6] CLINICAL SIGNS OF FAMILIAL HYPERCHOLESTEROLEMIA IN PATIENTS WITH FAMILIAL DEFECTIVE APOLIPOPROTEIN-B-100 AND NORMAL LOW-DENSITY-LIPOPROTEIN RECEPTOR FUNCTION
MYANT, NB
GALLAGHER, JJ
KNIGHT, BL
MCCARTHY, SN
FROSTEGARD, J
NILSSON, J
HAMSTEN, A
TALMUD, P
HUMPHRIES, SE
ARTERIOSCLEROSIS AND THROMBOSIS, 1991, 11 (03): : 691 - 703
[7] IDENTIFICATION OF THE LOW-DENSITY LIPOPROTEIN (LDL) RECEPTOR-BINDING DOMAIN OF HUMAN APOLIPOPROTEIN-B-100 (APOB-100)
HOSPATTANKAR, AV
LAW, SW
LACKNER, KJ
BREWER, HB
CIRCULATION, 1985, 72 (04) : 10 - 10
[8] LOCATING A LOW-DENSITY LIPOPROTEIN-TARGETING DOMAIN OF HUMAN APOLIPOPROTEIN-B-100 BY EXPRESSING A MINIGENE CONSTRUCT IN TRANSGENIC MICE
XIONG, WJ
ZSIGMOND, E
GOTTO, AM
LEI, KY
CHAN, L
JOURNAL OF BIOLOGICAL CHEMISTRY, 1991, 266 (31) : 20893 - 20898
[9] SECONDARY STRUCTURE AND DOSAGE OF SOLUBLE AND MEMBRANE-PROTEINS BY ATTENUATED TOTAL REFLECTION FOURIER-TRANSFORM INFRARED-SPECTROSCOPY ON HYDRATED FILMS
GOORMAGHTIGH, E
CABIAUX, V
RUYSSCHAERT, JM
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1990, 193 (02): : 409 - 420
[10] EFFECT OF LOW-DENSITY-LIPOPROTEIN RECEPTOR DEFICIENCY ON THE METABOLISM OF APOLIPOPROTEIN-B-100 IN BLOOD-PLASMA - KINETIC-STUDIES IN NORMAL AND WATANABE HERITABLE HYPERLIPIDEMIC RABBITS
YAMADA, N
SHAMES, DM
HAVEL, RJ
JOURNAL OF CLINICAL INVESTIGATION, 1987, 80 (02): : 507 - 515

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