ENHANCEMENT OF IMMUNOASSAY SENSITIVITY BY MOLECULAR MODIFICATION OF COMPETITORS

被引：17

作者：

JOCKERS, R ^{[1
]}

BIER, FF ^{[1
]}

SCHMID, RD ^{[1
]}

机构：

[1] GESELL BIOTECHNOL FORSCH MBH,PROJECT GRP BIOSENSORS,W-3300 BRAUNSCHWEIG,GERMANY

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1993年 / 163卷 / 02期

关键词：

ATRAZINE DETERMINATION; FIBER OPTICAL SENSOR; WEAK COMPETITOR; MONOCLONAL ANTIBODY;

D O I：

10.1016/0022-1759(93)90118-Q

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The potential of weak competitors to enhance the sensitivity of competitive immunoassays is described. Several triazine derivatives have been analyzed for their use as competitors. Their binding properties were determined using an enzyme-linked immunosorbent assay (ELISA) with a monoclonal antibody specific to triazines. Selected derivatives were immobilized onto the surface of a fibre optic sensor and atrazine was determined in a competitive manner using a fluorescein-labelled antibody. Using the weak binding competitor 11-(4-ethylamino-6-methylthio-s-triazine-2-yl)undecanoic acid (TE11S) the detection limit for atrazine could be lowered 100-fold in comparison to 2-aminohexylamino-4-ethyl-amino-6-isopropylamino-s-triazine (AHA), the previously used competitor.

引用

页码：161 / 167

页数：7

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