RECOMBINANT PLASMID GENE-TRANSFER IN AMENDED SOIL

被引：9

作者：

SELVARATNAM, S ^{[1
]}

GEALT, MA ^{[1
]}

机构：

[1] DREXEL UNIV,DEPT BIOSCI & BIOTECHNOL,32ND & CHESTNUT ST,PHILADELPHIA,PA 19104

来源：

WATER RESEARCH | 1992年 / 26卷 / 01期

关键词：

WASTE-WATER; CONJUGATION; SOIL; ESCHERICHIA-COLI; PLASMID PHSV106; MOBILIZATION; MOB GENES; GENETICALLY ENGINEERED MICROORGANISMS (GEMS); GENE TRANSFER;

D O I：

10.1016/0043-1354(92)90108-G

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Genetically engineered E. coli amended into a non-sterile soil microcosm were able to mobilize their recombinant plasmid DNA into a plasmidless E. coli recipient. This transfer required participation of a mobilizer bacterium containing a self-transmissible (conjugative) plasmid. Mobilization also depended on mob sequences present on the non-conjugative recombinant plasmid. Sequences from the non-conjugative plasmid pHSV106, which contains the herpes simplex virus thymidine kinase gene, were identified by DNA hybridization in recipient cells isolated from the soil.

引用

页码：39 / 43

页数：5

共 24 条

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