ISOLATION OF AN ADDITIONAL MEMBER OF THE FIBROBLAST GROWTH-FACTOR RECEPTOR FAMILY, FGFR-3

被引：441
作者：
KEEGAN, K
JOHNSON, DE
WILLIAMS, LT
HAYMAN, MJ
机构：
[1] SUNY STONY BROOK,DEPT MICROBIOL,STONY BROOK,NY 11794
[2] UNIV CALIF SAN FRANCISCO,CARDIOVASC RES INST,PROGRAM EXCELLENCE MOLEC BIOL,SAN FRANCISCO,CA 95143
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 04期
关键词：
TYROSINE KINASE GROWTH FACTOR RECEPTOR; ACTIVATION BY ACIDIC AND BASIC FIBROBLAST GROWTH FACTORS; K-562; CELLS; CDNA;
D O I：
10.1073/pnas.88.4.1095
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The fibroblast growth factors are a family of polypeptide growth factors involved in a variety of activities including mitogenesis, angiogenesis, and wound healing. Fibroblast growth factor receptors (FGFRs) have previously been identified in chicken, mouse, and human and have been shown to contain an extracellular domain with either two or three immunoglobulin-like domains, a transmembrane domain, and a cytoplasmic tyrosine kinase domain. We have isolated a human cDNA for another tyrosine kinase receptor that is highly homologous to the previously described FGFR. Expression of this receptor cDNA in COS cells directs the expression of a 125-kDa glycoprotein. We demonstrate that this cDNA encodes a biologically active receptor by showing that human acidic and basic fibroblast growth factors activate this receptor as measured by Ca-45(2+) efflux assays. These data establish the existence of an additional member of the FGFR family that we have named FGFR-3.
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页码：1095 / 1099
页数：5
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