IDENTIFICATION OF A CATALYTICALLY ESSENTIAL NUCLEOPHILIC RESIDUE IN SHEEP LIVER CYTOPLASMIC ALDEHYDE DEHYDROGENASE

被引：37

作者：

KITSON, TM ^{[1
]}

HILL, JP ^{[1
]}

MIDWINTER, GG ^{[1
]}

机构：

[1] UNIV HULL,DEPT BIOCHEM,HULL HU6 7RX,N HUMBERSIDE,ENGLAND

来源：

BIOCHEMICAL JOURNAL | 1991年 / 275卷

关键词：

D O I：

10.1042/bj2750207

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sheep liver cytoplasmic aldehyde dehydrogenase was labelled by reaction with the substrate p-nitrophenyl di[C-14]methylcarbamate. After tryptic digestion and peptide fractionation the labelled residue was identified as Cys-302. This is the first unequivocal identification of the essential enzymic nucleophile in the esterase activity of aldehyde dehydrogenase. By implication, Cys-302 is probably also the residue that is acylated by aldehyde substrates and the first residue that is modified by disulfiram.

引用

页码：207 / 210

页数：4

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