A COMPARISON OF THE SEQUENCES OF SEGMENT-A OF 4 INFECTIOUS BURSAL DISEASE VIRUS-STRAINS AND IDENTIFICATION OF A VARIABLE REGION IN VP2

被引：271

作者：

BAYLISS, CD ^{[1
]}

SPIES, U ^{[1
]}

SHAW, K ^{[1
]}

PETERS, RW ^{[1
]}

PAPAGEORGIOU, A ^{[1
]}

MULLER, H ^{[1
]}

BOURSNELL, MEG ^{[1
]}

机构：

[1] UNIV GIESSEN,INST VIROL,W-6300 GIESSEN,GERMANY

来源：

JOURNAL OF GENERAL VIROLOGY | 1990年 / 71卷

关键词：

D O I：

10.1099/0022-1317-71-6-1303

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The nucleotide sequences of the large open reading frame (ORF) from segment A of three European strains of infectious bursal disease virus (IBDV) have been determined using cDNA clones. This ORF of 3036 nucleotides encodes the virion proteins as a polyprotein in the following order: VP2, VP4, VP3. The nucleotide sequences determined have been compared to each other and to the published sequence of an Australian strain. The four strains are closely related, the greatest difference between two strains being 7.7% at the nucleotide level and 2.7% at the amino acid level. Comparisons show that there is a tight cluster of amino acid changes in the virion protein VP2. This variable region corresponded to the region where binding of a neutralizing monoclonal antibody has previously been mapped. A region in the centre of the segment, corresponding to the N terminal of VP4, was found to be completely conserved. Amino acid changes were spread fairly evenly through VP3 and there was no indication of a variable region as found in VP2.

引用

页码：1303 / 1312

页数：10

共 38 条

[1] DELETION MAPPING AND EXPRESSION IN ESCHERICHIA-COLI OF THE LARGE GENOMIC SEGMENT OF A BIRNAVIRUS [J].