REQUIREMENT OF ETS-2 EXPRESSION FOR XENOPUS OOCYTE MATURATION

被引：41

作者：

CHEN, ZQ ^{[1
]}

BURDETT, LA ^{[1
]}

SETH, AK ^{[1
]}

LAUTENBERGER, JA ^{[1
]}

PAPAS, TS ^{[1
]}

机构：

[1] NCI,MOLEC ONCOL LAB,FREDERICK,MD 21701

来源：

SCIENCE | 1990年 / 250卷 / 4986期

关键词：

D O I：

10.1126/science.2255913

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A molecular clone of the Xenopus laevis ets-2 gene was isolated from an oocyte complementary DNA library. The amount of messenger RNA (mRNA) in each oocyte or embryo was almost constant during oogenesis and was maintained until the blastula stage of embryonic development, indicating that the observed 3.2-kilobase transcript is a maternal message. The only normal adult tissue in which ets-2 mRNA was detected was the ovary. Injection of antisense oligonucleotides homologous to the ets-2 sequence into oocytes led to degradation of the mRNA and blocked hormone-induced germinal vesicle breakdown. The ets-2 product is thus required for the meiotic maturation of Xenopus oocytes.

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页码：1416 / 1418

页数：3

相关论文

共 32 条

[1] TS MUTANTS OF E26 LEUKEMIA-VIRUS ALLOW TRANSFORMED MYELOBLASTS, BUT NOT ERYTHROBLASTS OR FIBROBLASTS, TO DIFFERENTIATE AT THE NONPERMISSIVE TEMPERATURE [J].

BEUG, H ;

LEUTZ, A ;

KAHN, P ;

GRAF, T .

CELL, 1984, 39 (03) :579-588

[2] MYELOBLASTS TRANSFORMED BY THE AVIAN ACUTE-LEUKEMIA VIRUS E26 ARE HORMONE-DEPENDENT FOR GROWTH AND FOR THE EXPRESSION OF A PUTATIVE MYB-CONTAINING PROTEIN,P135 E26 [J].

BEUG, H ;

HAYMAN, MJ ;

GRAF, T .

EMBO JOURNAL, 1982, 1 (09) :1069-1073

[3] IDENTIFICATION IN CHICKENS OF AN EVOLUTIONARILY CONSERVED CELLULAR ETS-2 GENE (C-ETS-2) ENCODING NUCLEAR PROTEINS RELATED TO THE PRODUCTS OF THE C-ETS PROTO-ONCOGENE [J].

BOULUKOS, KE ;

POGNONEC, P ;

BEGUE, A ;

GALIBERT, F ;

GESQUIERE, JC ;

STEHELIN, D ;

GHYSDAEL, J .

EMBO JOURNAL, 1988, 7 (03) :697-705

[4]

BURDETT LA, UNPUB

[5] MOLECULAR-CLONING OF THE ETS-PROTO-ONCOGENE OF THE SEA-URCHIN AND ANALYSIS OF ITS DEVELOPMENTAL EXPRESSION [J].

CHEN, ZQ ;

KAN, NC ;

PRIBYL, L ;

LAUTENBERGER, JA ;

MOUDRIANAKIS, E ;

PAPAS, TS .

DEVELOPMENTAL BIOLOGY, 1988, 125 (02) :432-440

[6] SELECTIVE ELIMINATION OF MESSENGER-RNAS INVIVO - COMPLEMENTARY OLIGODEOXYNUCLEOTIDES PROMOTE RNA DEGRADATION BY AN RNASE H-LIKE ACTIVITY [J].

DASH, P ;

LOTAN, I ;

KNAPP, M ;

KANDEL, ER ;

GOELET, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (22) :7896-7900

[7] OOGENESIS IN XENOPUS-LAEVIS (DAUDIN) .1. STAGES OF OOCYTE DEVELOPMENT IN LABORATORY MAINTAINED ANIMALS [J].

DUMONT, JN .

JOURNAL OF MORPHOLOGY, 1972, 136 (02) :153-&

[8]

FUJIWARA S, 1988, ONCOGENE, V2, P99

[9] A SHORT-LIVED NUCLEAR PHOSPHOPROTEIN ENCODED BY THE HUMAN ETS-2 PROTO-ONCOGENE IS STABILIZED BY ACTIVATION OF PROTEIN KINASE-C [J].

FUJIWARA, S ;

FISHER, RJ ;

BHAT, NK ;

DELAESPINA, SMD ;

PAPAS, TS .

MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (11) :4700-4706

[10] TRANSFORMING CAPACITIES AND DEFECTIVENESS OF AVIAN LEUKEMIA VIRUSES OK10 AND E26 [J].

GRAF, T ;

OKERBLOM, N ;

TODOROV, TG ;

BEUG, H .

VIROLOGY, 1979, 99 (02) :431-436

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