INFLUENCE OF CULTURAL AND PHYSIOCHEMICAL FACTORS ON ASCORBATE STABILITY IN PLANT-TISSUE CULTURE MEDIA

被引：9
作者：
ELMORE, HW
SAMPLES, B
SHARMA, S
HARRISON, M
机构：
[1] Department of Biological Sciences, Marshall University, Huntington, 25701, WV
来源：
PLANT CELL TISSUE AND ORGAN CULTURE | 1990年 / 20卷 / 02期
关键词：
dehydroascorbic acid; L-ascorbic aid; medium; tissue culture;
D O I：
10.1007/BF00114711
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Ascorbic acid rapidly decays in plant tissue culture media. Within 50 min to 3 h after preparing 100 mM solutions, ascorbic acid was destroyed. Autoclaving, shaking flasks, high light intensity and increasing pH over a range from 4.5-7 accelerated decay. Ascorbic acid was oxidized to dehydroascorbic acid which also underwent decay. Within 11 h and 15 min after adding ascorbic acid both ascorbic acid and its oxidation product, dehydroascorbic acid, disappeared from medium. Since ascorbic acid is rapidly destroyed in plant tissue culture media it may not exert its effect as an intact molecule. Instead its antioxidant/antibrowning role in plant cell, tissue and organ cultures may be mediated by some product of further oxidation. © 1990 Kluwer Academic Publishers.
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页码：131 / 135
页数：5
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