PROLINE REDUCTASE - SENSITIVE FLUOROMETRIC ASSAY WITH ORTHO-PHTHALALDEHYDE

被引：10
作者：
SETO, B
机构：
[1] Laboratory of Biochemistry, National Heart, Lung and Blood Institute, Bethesda
来源：
ANALYTICAL BIOCHEMISTRY | 1979年 / 95卷 / 01期
关键词：
D O I：
10.1016/0003-2697(79)90183-0
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A rapid and sensitive fluorometric assay for measuring the activity of proline reductase is described. The product of the enzymic reaction, δ-aminovalerate, is converted to a highly fluorescent derivative by reaction with o-phthalaldehyde. The water-soluble fluorescent product exhibits an excitation maximum at 340 nm and an emission maximum at 455 nm. The fluorophor reacts specifically with δ-aminovalerate without any interference from proline. © 1979.
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页码：44 / 47
页数：4
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