A SERINE (THREONINE) PROTEIN-KINASE CONFERS FUNGICIDE RESISTANCE IN THE PHYTOPATHOGENIC FUNGUS USTILAGO-MAYDIS

被引：44
|
作者：
ORTH, AB
RZHETSKAYA, M
PELL, EJ
TIEN, M
机构：
[1] PENN STATE UNIV,DEPT BIOCHEM & MOLEC BIOL,UNIVERSITY PK,PA 16802
[2] PENN STATE UNIV,DEPT PLANT PATHOL,UNIVERSITY PK,PA 16802
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1995年 / 61卷 / 06期
关键词：
D O I：
10.1128/AEM.61.6.2341-2345.1995
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A mutant of Ustilago maydis (VR43) with single-gene resistance to the dicarboximide fungicide vinclozolin was previously isolated and characterized. A genomic library was constructed, and an 8.7-kb resistance-conferring fragment was isolated by sib selection. Sequencing this fragment, we identified an 1,218-bp open reading frame, which, if disrupted by deletion, no longer confers resistance. Analyses of the data in GenBank demonstrated a high degree of homology between the product of the 1,218-bp open reading frame, referred to as the adr-1 gene, and Ser (Thr) protein kinases.
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页码：2341 / 2345
页数：5
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