IMPROVED DETECTION LIMITS FOR ELECTROSPRAY-IONIZATION ON A MAGNETIC-SECTOR MASS-SPECTROMETER BY USING AN ARRAY DETECTOR

被引:14
|
作者
CODY, RB
TAMURA, J
FINCH, JW
MUSSELMAN, BD
机构
[1] JEOL USA, Inc., Peabody, 01960, MA
关键词
D O I
10.1016/1044-0305(94)85033-X
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Array detection was compared with point detection for solutions of hen egg-white lysozyme, equine myoglobin, and ubiquitin analyzed by electrospray ionization with a magnetic sector mass spectrometer. The detection limits for samples analyzed by using the array detector system were at least 10 times lower than could be achieved by using a point detector on the same mass spectrometer. The minimum detectable quantity of protein corresponded to a signal-to-background ratio of approximately 2:1 for a 500 amol/muL solution of hen egg-white lysozyme. However, the ultimate practical sample concentrations appeared to be in the 10-100 fmol/muL range for the analysis of dilute solutions of relatively pure proteins or simple mixtures.
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页码:194 / 200
页数:7
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