SOMATIC EMBRYOGENESIS IN CULTURED MATURE ZYGOTIC EMBRYOS OF ABIES-BALSAMEA

被引：20
|
作者：
GUEVIN, TG [1 ]
MICAH, V [1 ]
KIRBY, EG [1 ]
机构：
[1] RUTGERS STATE UNIV,DEPT BIOL SCI,NEWARK,NJ 07102
来源：
PLANT CELL TISSUE AND ORGAN CULTURE | 1994年 / 37卷 / 02期
关键词：
CONIFERS; CYTOKININS;
D O I：
10.1007/BF00043618
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Embryo suspensor masses (ESMs) were induced by culture of isolated mature zygotic embryos of balsam fir [Abies balsamea (Mill.)] on media containing 10 mu M cytokinin [6-(gamma-gamma-dimethylallylamino)purine (2iP), 6-benzyladenine (BA), or thidiazuron (TDZ)]. Once induced, ESMs proliferated on media containing 2iP, BA or TDZ (10 mu M) or on 4. 5 mu M BA in combination with 10 mu M naphthyl-1-acetic acid. When ESMs were transferred to media containing 5-80 mu M abscisic acid, cotyledonary-stage embryos were formed. Embryos were readily germinated on medium lacking growth regulators.
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页码：205 / 208
页数：4
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