SOMATIC EMBRYOGENESIS IN CULTURED MATURE ZYGOTIC EMBRYOS OF ABIES-BALSAMEA

被引：20

作者：

GUEVIN, TG ^{[1
]}

MICAH, V ^{[1
]}

KIRBY, EG ^{[1
]}

机构：

[1] RUTGERS STATE UNIV,DEPT BIOL SCI,NEWARK,NJ 07102

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 1994年 / 37卷 / 02期

关键词：

CONIFERS; CYTOKININS;

D O I：

10.1007/BF00043618

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Embryo suspensor masses (ESMs) were induced by culture of isolated mature zygotic embryos of balsam fir [Abies balsamea (Mill.)] on media containing 10 mu M cytokinin [6-(gamma-gamma-dimethylallylamino)purine (2iP), 6-benzyladenine (BA), or thidiazuron (TDZ)]. Once induced, ESMs proliferated on media containing 2iP, BA or TDZ (10 mu M) or on 4. 5 mu M BA in combination with 10 mu M naphthyl-1-acetic acid. When ESMs were transferred to media containing 5-80 mu M abscisic acid, cotyledonary-stage embryos were formed. Embryos were readily germinated on medium lacking growth regulators.

引用

页码：205 / 208

页数：4

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