A SIMPLE AND INEXPENSIVE GLUCOSE-OXIDASE SUBSTRATE SYSTEM FOR ENZYME-IMMUNOASSAY

被引：6

作者：

BLAIS, BW

YAMAZAKI, H

机构：

[1] CARLETON UNIV,DEPT BIOL,OTTAWA K1S 5B6,ONTARIO,CANADA

[2] CARLETON UNIV,INST BIOCHEM,OTTAWA K1S 5B6,ONTARIO,CANADA

来源：

IMMUNOLOGICAL INVESTIGATIONS | 1992年 / 21卷 / 06期

基金：

加拿大自然科学与工程研究理事会;

关键词：

D O I：

10.3109/08820139209069392

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A simple and inexpensive glucose oxidase substrate system was developed for enzyme immunoassay. This system is based on the formation of a coloured complex between polyvinyl alcohol or starch and iodine produced from iodide in the presence of hydrogen peroxide generated by the glucose oxidase reaction. The rate of iodine production was enhanced by the supplementation of molybdate. In an enzyme immunoassay for anti-Salmonella antibodies using glucose oxidase as the indicator enzyme, the molybdate-enhanced polyvinyl alcohol- and starch-glucose-iodide substrate systems were as sensitive as a conventional glucose oxidase assay system employing horseradish peroxidase as a secondary enzyme and a suitable hydrogen-donating chromogen.

引用

页码：581 / 588

页数：8

共 11 条

[1]

AVRAMEAS S, 1978, SCAND J IMMUNOL S7, V8, P7

[2]

Bergmeyer H.U., 1983, METHOD ENZYMAT AN, V2, P201

[3]

BLAIS B W, 1989, Biotechnology Techniques, V3, P253, DOI 10.1007/BF01876059

[4]

Bos E S, 1981, J Immunoassay, V2, P187, DOI 10.1080/15321818108056977