BOVINE ADENOVIRUSES .4. 2 MIXED ANTIGENS FOR ROUTINE SERODIAGNOSIS BY COMPLEMENT-FIXATION REACTION

被引:4
作者
MESSNER, A
HINAIDY, B
BURKI, F
机构
[1] Institute of Virology, Veterinary University, A 1030 Vienna
关键词
Bovine adenoviruses; complement fixation reaction; mixed antigens; subgroups I and II;
D O I
10.1016/0147-9571(79)90036-5
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The frequency of bovine adenovirus infections occurring in cattle has been grossly underestimated up to the present day. Primary isolation of serotypes belonging to bovine subgroup II is cumbersome. Hardly any laboratory has used all 8 officially-recognised bovine serotypes in seroneutralisation-tests, which should be done when this type-specific method is used for serodiagnosis. The complement fixation test, known as group-reactive from human adenovirus serology, has failed to disclose the true incidence of bovine infections, as until recently the importance of a novel additional group-reactive bovine adenovirus antigen had been undisclosed. Here we describe production, composition and performance of two mixed antigens for complement fixation reactions, which take into account recent findings by our team on peculiarities of bovine adenovirus antigens. Mixed antigen 1 contains bovine serotypes 1, 2 and 3 and detects group-specific antibodies of the classical mastadenoviruses. Mixed antigen 2 contains bovine serotypes 4, 5, 6, 7 and 8 and determines group-specific antibodies against the novel paramastadenoviruses. In addition, each antigen is capable of demonstrating complement-fixing type-specific antibodies in sera against the respective types included in each mixed antigen, with a net enhancing effect produced by mixing. Use of the 2 mixed antigens promptly shows whether bovine adenoviruses, presently recognised as well as unclassified, are involved in a given outbreak of respiratory or enteric disease of cattle. If needed, the responsible type may be determined in a second step of serological investigation. © 1979.
引用
收藏
页码:351 / 359
页数:9
相关论文
共 29 条
[1]  
BARTHA A, 1969, ACTA VET ACAD SCI H, V19, P319
[2]  
BINN LN, 1960, J IMMUNOL, V84, P20
[3]  
Bockmann J., 1976, Wiener Tierarztliche Monatsschrift, V63, P117
[4]  
BOCKMANN J, 1973, THESIS U WIEN
[5]  
BURKI F, 1979, MICROBIOLOGICA, V2, P65
[6]  
BURKI F, 1978, ZBL VET MED B, V25, P555
[7]  
BURKI F, 1973, J AM VET MED ASSOC, V163, P897
[8]  
BURKI F., 1960, Zeitschrift fur Immunitatsforschung und Experimentelle Therapie, V119, P320
[9]  
CASEY HL, 1965, HEW74 PUBL HLTH MON, P34
[10]  
DOHNER L, 1968, Z IMMUNITATSFORSCH, V136, P451