KINETICS OF PHOTOCHEMICAL ADDITION OF [35S]CYSTEINE TO POLYNUCLEOTIDES AND NUCLEIC ACIDS

被引：61

作者：

SMITH, KC

MEUN, DHC

机构：

[1] Department of Radiology, Stanford University School of Medicine, Palo Alto

来源：

BIOCHEMISTRY | 1968年 / 7卷 / 03期

关键词：

D O I：

10.1021/bi00843a023

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

[35S]Cysteine was ultraviolet irradiated in the presence of poly rU, poly rU:rA, poly rA, poly rC, poly dC, poly dC:dG, poly dT, poly dA:dT, poly dAT :dAT, ribonucleic acid (RNA), or deoxyribonucleic acid (DNA), and the rate constants for the uptake of cysteine per dose of ultraviolet light were tabulated. Whether the polymers were single or double stranded (and/or protonated) had a profound effect upon the reaction rate. Preliminary experiments indicate that tyrosine and serine also add photochemically to DNA but threonine and methionine appear unreactive under the conditions used. These photochemical reactions may provide the mechanism by which DNA and protein are crosslinked in vivo when cells are irradiated with ultraviolet light. © 1968, American Chemical Society. All rights reserved.

引用

页码：1033 / &

共 29 条

[1]

BOLLUM FJ, 1966, PROCEDURES NUCLEIC A, P577

[2]

BOWEN EJ, 1946, CHEMICAL ASPECTS LIG, P282

[3] EFFECT OF SOLUTION COMPOSITION UPON POLY U1 [J].

BROWN, RA .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1966, 115 (01) :102-&

[4]

BURR JG, 1967, RADIAT RES, V31, P547

[5]

CANTONI GL, 1966, PROCEDURES NUCLEI ED, P577

[6]

CHARGAFF E, 1955, NUCLEIC ACIDS ED, V1, P211

[7]

Cohn E. J., 1943, PROTEINS AMINO ACIDS

[8]

COHN WE, 1955, NUCLEIC ACIDS, V1, P211

[9]

DEERING RA, 1963, BIOCHIM BIOPHYS ACTA, V68, P526

[10] STUDIES ON THE FORMATION OF 2-STRANDED AND 3-STRANDED POLYRIBONUCLEOTIDES [J].

FELSENFELD, G ;

RICH, A .

BIOCHIMICA ET BIOPHYSICA ACTA, 1957, 26 (03) :457-468

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